A SUBPOPULATION OF SMOOTH-MUSCLE CELLS IN INJURED RAT ARTERIES EXPRESSES PLATELET-DERIVED GROWTH FACTOR-B CHAIN MESSENGER-RNA
A SUBPOPULATION OF SMOOTH-MUSCLE CELLS IN INJURED RAT ARTERIES EXPRESSES PLATELET-DERIVED GROWTH FACTOR-B CHAIN MESSENGER-RNA
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DOI：
10.1161/01.res.76.6.951
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发表时间：
1995-06-01
影响因子：
20.1
通讯作者：
REIDY, MA
中科院分区：
文献类型：
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作者：
LINDNER, V;GIACHELLI, CM;REIDY, MA
平滑肌细胞增殖和新生内膜形成是大鼠颈动脉对球囊损伤反应的特征。大鼠血小板衍生生长因子（PDGF）-B的克隆，从而使我们能够使用物种特异性探针进行原位杂交损伤的动脉表面。在新生内膜的发育过程中，管腔SMC（7%至10%）表达PDGF-B mRNA，但当病变停止生长时，极少数管腔SMC仍表达PDGF-B（0.5%）。原代培养的SMC中，1.6%的正常图尼卡中膜SMC表达PDGF-B mRNA，11%的新生内膜SMC表达PDGF-B mRNA。这些数据表明，SMC在受伤的血管壁是异质性的PDGF-B的表达和这些细胞的传代培养可能会产生的文化，无论是积极的或消极的PDGF-B的表达。此外，与丰富的表达的PDGF受体β亚基表达的内膜SMC，我们的研究结果提供了证据，这些细胞合成的PDGF-B可能通过旁分泌或自分泌机制参与内膜病变的形成。
Proliferation of smooth muscle cells (SMCs) and formation of a neointima are characteristics of the response of rat carotid arteries to balloon injury. Rat platelet-derived growth factor (PDGF)-B was cloned, thus allowing us to use species-specific probes to carry out in situ hybridization on the surface of injured arteries. A distinct population of luminal SMCs (7% to 10%) in the developing neointima expressed PDGF-B mRNA, but very few luminal SMCs still expressed PDGF-B (0.5%) when the lesion had stopped growing. Primary SMC cultures revealed expression of PDGF-B mRNA in 1.6% of SMCs derived from normal tunica media and in 11% of SMCs derived from the neointima. These data demonstrate that SMCs in the injured vessel wall are heterogeneous with regard to PDGF-B expression and that subculturing of these cells may give rise to cultures that are either positive or negative for PDGF-B expression. Furthermore, with abundant expression of the PDGF receptor beta-subunit expressed by intimal SMCs, our findings provide evidence that PDGF-B synthesized by these cells may be involved in intimal lesion formation via a paracrine or autocrine mechanism.