Preparation of a biomimetic polyphosphorylcholine monolithic column for immobilized artificial membrane chromatography.
Preparation of a biomimetic polyphosphorylcholine monolithic column for immobilized artificial membrane chromatography.
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DOI：
10.1016/j.chroma.2015.06.056
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发表时间：
2015-08
期刊：
影响因子：
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通讯作者：
Xianglong Zhao;Weijia Chen;Zhengyin Zhou;Qiqin Wang;Zhenghua Liu;R. Moaddel;Zhengjin Jiang
中科院分区：
文献类型：
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作者：
Xianglong Zhao;Weijia Chen;Zhengyin Zhou;Qiqin Wang;Zhenghua Liu;R. Moaddel;Zhengjin Jiang
The present work aims to prepare a novel phosphatidylcholine functionalized monolithic stationary phase byin situco-polymerization of 12-methacryloyl dodecylphosphocholine (MDPC) and ethylene dimethacrylate (EDMA) for immobilized artificial membrane chromatography. Scanning electron microscopy, energy dispersive X-ray spectroscopy, FT-IR spectroscopy, pore size distribution analysis, ζ-potential analysis and micro-HPLC were used to evaluate the monolithic structure and physicochemical properties. Satisfactory morphology, high mechanical stability, good permeability and chromatographic performance were obtained on the optimized monolithic columns. A typical reverse-phase retention mechanism was observed over a wide range of organic solvent content (acetonitrile< 80%). The optimized poly(MDPC-co-EDMA) monolith exhibited good selectivity for proteins and basic drugs. Good correlation was observed between the retention on commercial IAM column (IAM.PC.DD2) and poly(MDPC-co-EDMA) monolith. This novel poly(MDPC-co-EDMA) monolith exhibited good potential for studying the drug-membrane interaction.
