An injectable BMSC-laden enzyme-catalyzed crosslinking collagen-hyaluronic acid hydrogel for cartilage repair and regeneration
An injectable BMSC-laden enzyme-catalyzed crosslinking collagen-hyaluronic acid hydrogel for cartilage repair and regeneration
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一种可注射的 BMSC 负载酶催化交联胶原-透明质酸水凝胶，用于软骨修复和再生
DOI：
10.1039/d0tb00291g
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发表时间：
2020
影响因子：
7
通讯作者：
Pei Renjun
中科院分区：
文献类型：
--
作者：
Zhang Yajie;Cao Yi;Zhao Hongbo;Zhang Liwei;Ni Tianyu;Liu Yuanshan;An Zhen;Liu Min;Pei Renjun
关节软骨由于缺乏丰富的营养和祖细胞，自我修复能力有限。在这项研究中，一种可注射的水凝胶系统组成的I型胶原蛋白-酪胺（Col-TA）和透明质酸-酪胺（HA-TA）作为骨髓间充质干细胞（BMSC）负载的水凝胶系统的软骨再生。接下来，该水凝胶系统的理化性能进行了表征和优化，包括凝胶化时间，刚度，吸水性和降解性。进一步评价BMSCs在Col-HA水凝胶中的增殖和分化，并在转化生长因子-β1（TGF-β1）存在下检测体内软骨修复能力。这些结果表明，这种水凝胶可以提供一个伟大的微环境，BMSC的生长和软骨分化在体外和体内，和Col-HA水凝胶可以作为一个理想的水凝胶软骨组织再生。
Articular cartilage has limited self-healing ability due to its lack of abundant nutrients and progenitor cells. In this study, an injectable hydrogel system consisting of collagen type I-tyramine (Col-TA) and hyaluronic acid-tyramine (HA-TA) was fabricated as the bone marrow mesenchymal stem cell (BMSC)-laden hydrogel system for cartilage regeneration. Next, the physiochemical properties of this hydrogel system were well characterized and optimized, including gelation time, stiffness, water absorption and degradability. Further, the proliferation and differentiation of BMSCs within the Col-HA hydrogel were evaluated, and the ability of in vivo cartilage repair was also examined in the presence of the transforming growth factor-β1 (TGF-β1). These results illustrate that this hydrogel can offer a great microenvironment for BMSC growth and cartilage differentiation both in vitro and in vivo, and the Col-HA hydrogel can serve as an ideal hydrogel for cartilage tissue regeneration.