Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane
Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane
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DOI：
10.1039/b717900f
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Han, Jongyoon
中科院分区：
文献类型：
--
作者：
Lee, Jeong Hoon;Song, Yong-Ak;Han, Jongyoon
本文报道了一种以聚二甲基硅氧烷（PDMS）微流控芯片形式制备高通量蛋白质预浓缩剂的新方法。我们使用标准的图像化技术在玻璃基板上印刷了一层亚微米厚的离子选择膜。通过在印刷玻璃基板上简单的等离子体键合PDMS微流控装置，我们可以将离子选择膜集成到器件中，并且无需复杂的微加工和繁琐的集成过程即可快速原型化PDMS预浓缩器。PDMS预富集器在5 min内的富集系数高达10(4)。这种打印方法甚至允许制造平行阵列的预浓缩器，以增加浓缩样品的体积，这可以促进我们的微流控预浓缩器芯片的集成，作为信号增强工具到各种探测器，如质谱仪。
In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as similar to 10(4) in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.