Genome-wide DNA methylation association study of recent and cumulative marijuana use in middle aged adults.
Genome-wide DNA methylation association study of recent and cumulative marijuana use in middle aged adults.
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DOI：
10.1038/s41380-023-02106-y
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发表时间：
2023-06
影响因子：
11
通讯作者：
Hou, Lifang
中科院分区：
文献类型：
--
作者：
Nannini, Drew R. R.;Zheng, Yinan;Joyce, Brian T.;Kim, Kyeezu;Gao, Tao;Wang, Jun;Jacobs, David R. R.;Schreiner, Pamela J. J.;Yaffe, Kristine;Greenland, Philip;Lloyd-Jones, Donald M. M.;Hou, Lifang
大麻在美国是一种广泛使用的精神活性物质，医疗和娱乐合法化在过去十年中有所上升。尽管吸食大麻的人越来越多，但调查表观遗传因素与最近和累计吸食大麻之间关系的研究仍然有限。因此，我们调查了近期和累计吸食大麻与DNA甲基化水平之间的关系。来自青年冠状动脉风险发展研究的参与者在检查年(Y)15和Y20收集了全血，被随机选择在这两个时间点使用Illumina甲基EPIC珠芯片进行DNA甲基化分析。在每次检查中都会询问最近的大麻使用情况，并用它来估计从Y0到Y15和Y20的累计大麻使用情况。在Y15(n = 1023)，我们分别观察到22和31个甲基化标记(FDR P ≤ 0.05)与最近和累积吸食大麻有关，在Y20(n = 883)分别观察到132和16个甲基化标记。我们复制了8个以前报道的与大麻使用相关的甲基化标记。我们进一步确定了640个cis-meQTL和198个dmr，它们与第15年和第20年的近期和累积使用有关。差异甲基化基因在与细胞增殖、激素信号和感染以及精神分裂症、双相情感障碍和物质相关障碍相关的途径中有统计上的过度表达。我们确定了许多与中年成年人最近和累积使用大麻有关的甲基化标记、途径和疾病，为大麻使用和表观基因组之间的关联提供了更多的洞察力。这些结果为大麻对表观基因组和相关健康状况的作用提供了新的见解。
Marijuana is a widely used psychoactive substance in the US and medical and recreational legalization has risen over the past decade. Despite the growing number of individuals using marijuana, studies investigating the association between epigenetic factors and recent and cumulative marijuana use remain limited. We therefore investigated the association between recent and cumulative marijuana use and DNA methylation levels. Participants from the Coronary Artery Risk Development in Young Adults Study with whole blood collected at examination years (Y) 15 and Y20 were randomly selected to undergo DNA methylation profiling at both timepoints using the Illumina MethylationEPIC BeadChip. Recent use of marijuana was queried at each examination and used to estimate cumulative marijuana use from Y0 to Y15 and Y20. At Y15 (n = 1023), we observed 22 and 31 methylation markers associated (FDR P ≤ 0.05) with recent and cumulative marijuana use and 132 and 16 methylation markers at Y20 (n = 883), respectively. We replicated 8 previously reported methylation markers associated with marijuana use. We further identified 640 cis-meQTLs and 198 DMRs associated with recent and cumulative use at Y15 and Y20. Differentially methylated genes were statistically overrepresented in pathways relating to cellular proliferation, hormone signaling, and infections as well as schizophrenia, bipolar disorder, and substance-related disorders. We identified numerous methylation markers, pathways, and diseases associated with recent and cumulative marijuana use in middle-aged adults, providing additional insight into the association between marijuana use and the epigenome. These results provide novel insights into the role marijuana has on the epigenome and related health conditions.
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DOI：
10.1016/s2215-0366(19)30048-3
发表时间：
2019-05
期刊：
The lancet. Psychiatry
影响因子：
--
作者：
Di Forti M;Quattrone D;Freeman TP;Tripoli G;Gayer-Anderson C;Quigley H;Rodriguez V;Jongsma HE;Ferraro L;La Cascia C;La Barbera D;Tarricone I;Berardi D;Szöke A;Arango C;Tortelli A;Velthorst E;Bernardo M;Del-Ben CM;Menezes PR;Selten JP;Jones PB;Kirkbride JB;Rutten BP;de Haan L;Sham PC;van Os J;Lewis CM;Lynskey M;Morgan C;Murray RM;EU-GEI WP2 Group
通讯作者：
EU-GEI WP2 Group
影响因子：
6.2
作者：
Autism Spectrum Disorders Working Group of The Psychiatric Genomics Consortium
通讯作者：
Autism Spectrum Disorders Working Group of The Psychiatric Genomics Consortium
影响因子：
30.8
作者：
Das, Sayantan;Forer, Lukas;Schoenherr, Sebastian;Sidore, Carlo;Locke, Adam E.;Kwong, Alan;Vrieze, Scott I.;Chew, Emily Y.;Levy, Shawn;McGue, Matt;Schlessinger, David;Stambolian, Dwight;Loh, Po-Ru;Iacono, William G.;Swaroop, Anand;Scott, Laura J.;Cucca, Francesco;Kronenberg, Florian;Boehnke, Michael;Abecasis, Goncalo R.;Fuchsberger, Christian
通讯作者：
Fuchsberger, Christian
影响因子：
5.7
作者：
Campagna MP;Xavier A;Lechner-Scott J;Maltby V;Scott RJ;Butzkueven H;Jokubaitis VG;Lea RA
通讯作者：
Lea RA
影响因子：
14.9
作者：
Buniello, Annalisa;MacArthur, Jacqueline A. L.;Parkinson, Helen
通讯作者：
Parkinson, Helen