Cytokeratin 14 is expressed in immature cells in rat taste buds
Cytokeratin 14 is expressed in immature cells in rat taste buds
复制标题
DOI：
10.1007/s10735-007-9151-0
复制
发表时间：
2008-04-01
影响因子：
3.2
通讯作者：
Emori, Yasufumi
中科院分区：
文献类型：
--
作者：
Asano-Miyoshi, Misaki;Hamamichi, Ryoko;Emori, Yasufumi
我们分析了味蕾细胞的分化，通过精确描述细胞角蛋白（CKs）8和14的表达谱与标记分子，包括5-溴-2 '-脱氧尿苷（BrdU）的标记注射。在大鼠轮廓乳头，细胞分裂观察到在基底层的上皮细胞表达CK 14和位于味蕾外。在BrdU注射后1天（第1天），祖细胞开始向顶面迁移并维持CK 14表达。另一方面，少数新分裂的细胞很少被整合到味蕾中，并且在第1天也保持CK 14表达。在味蕾中，在第2-3天发生CK亚型从CK 14到细胞角蛋白8（CK 8）的转化，显示从表达CK 14的未成熟细胞分化为表达CK 8的成熟或正在成熟的细胞。功能成熟的细胞，如表达三磷酸肌醇受体3型（IP（3）R3）的味觉感受器细胞从不表达CK 14，这表明CK 14只在未成熟的细胞中表达。另一方面，一个小的，但独特的人口BrdU阳性细胞仍然表现出CK 14免疫反应性的味蕾，甚至在第12天，这可能对应于细胞，保持未分化的味蕾内很长一段时间。
We analyzed the differentiation of taste bud cells, by precisely describing expression profiles of cytokeratins (CKs) 8 and 14 in relation to those of marker molecules including label of 5-bromo-2'-deoxy uridine (BrdU) injected. In rat circumvallate papillae, cell division was observed at the basal layer of the epithelium expressing CK14 and located outside taste buds. The progenitor cells began to migrate toward the apical surface and maintained CK14 expression at 1 day after BrdU injection (day 1). On the other hand, a minor population of newly divided cells was infrequently incorporated into taste buds and also maintained CK14 expression at day 1. In taste buds, the conversion of CK subtypes occurred from CK14 to cytokeratin 8 (CK8) at day 2-3, showing the differentiation from immature cells expressing CK14 into mature or maturing cells expressing CK8. Functionally matured cells such as taste receptor cells expressing inositol triphospate receptor type 3 (IP(3)R3) never expressed CK14, suggesting that CK14 would be expressed only in immature cells. On the other hand, a small but distinct population of BrdU-positive cells still showed CK14 immunoreactivity in taste buds even at day 12, which might correspond to the cells that remain undifferentiated for a long period within taste buds.