Immunolocalization of aquaporin-1 in the mechanoreceptive Ruffini endings in the periodontal ligament
Immunolocalization of aquaporin-1 in the mechanoreceptive Ruffini endings in the periodontal ligament
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DOI：
10.1016/j.brainres.2007.04.033
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发表时间：
2007-07-09
期刊：
影响因子：
2.9
通讯作者：
Maeda, Takeyasu
中科院分区：
文献类型：
--
作者：
Nandasena, Batheegama Gammacharige Tharanga Lakmali;Suzuki, Akiko;Maeda, Takeyasu
以前的超微结构研究表明，在牙周Ruffini结束，一个主要的机械感受器轴突Schwarm细胞的相互作用。然而，没有关于它们之间的传输机制的信息。本研究探讨水通道蛋白-1（AQP 1）和-4（AQP 4），水选择性通道的成员，在大鼠切牙和三叉神经节的牙周Ruffini末梢的免疫定位。此外，AQP 1和4的mRNA的表达在三叉神经节中检测通过RT-PCR技术。预期AQP 1和4的大小的单个PCR产物可在三叉神经节的逆转录cDNA样品中检测到，三叉神经节的神经元支配牙周Ruffini末梢。AQP 1的免疫反应是可识别的轴突终端的牙周Ruffini末梢以及其相关的终端许旺细胞，证实了与AQP 1和PGP9.5或S-100蛋白的双重染色。而牙周组织Ruffini终末未见AQP 4的表达。虽然AQP 4免疫反应只局限于三叉神经节的卫星细胞，但在三叉神经节的神经元中未见AQP 1免疫反应，但有16.1%的三叉神经节神经元显示AQP 1免疫反应。AQP 1阳性或阴性神经元周围的卫星细胞也有AQP 1免疫反应。对这些阳性神经元横截面积的分析表明，大约66.9%的阳性神经元为400-1000 μ m（2）（671.4 +/- 172.4 μ m（2）），表明它们可以被归类为介导机械传导的中等大小的神经元。这些结果表明，AQP 1控制水运输的牙周Ruffini结束。(c)2007 Elsevier B. V.保留所有权利。
Previous ultrastructural studies have suggested an axon-Schwarm cell interaction in the periodontal Ruffini ending, a primary mechanoreceptor. However, no information is available on the transport mechanism between them. The present study examined the immunolocalization of aquaporin-1 (AQP1) and -4 (AQP4), a member of the water-selective channel, in the periodontal Ruffini endings of the rat incisors and trigeminal ganglion. In addition, the expression of mRNA for AQP1 and 4 was detected in the trigeminal ganglion by a RT-PCR technique. A single PCR product of the sizes anticipated for AQP1 and 4 was detectable in a reverse transcripted cDNA sample from the trigeminal ganglion, whose neurons innervate the periodontal Ruffini endings. An AQP1 immunoreaction was recognizable in the axon terminals of the periodontal Ruffini endings as well as their associated terminal Schwann cells, as confirmed with a double staining with AQP1 and either PGP9.5 or S-100 protein. However, no immunoreaction for AQP4 was found in periodontal Ruffini endings. Although the AQP4 immunoreaction was localized in some satellite cells - but never in neurons - of the trigeminal ganglion, 16.1% trigeminal neurons showed the AQP1 immunoreaction. Furthermore, the AQP1 immunoreaction was found in certain satellite cells which surrounded AQP1-positive or -negative neurons. An analysis of a cross-sectional area of these positive neurons demonstrated that approximately 66.9% of the positive neurons were 400-1000 mu m(2) (671.4 +/- 172.4 mu m(2)), indicating that they could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that AQP1 controls water transport in the periodontal Ruffini endings. (c) 2007 Elsevier B.V. All rights reserved.