Enhancement of apoptosis by sonodynamic therapy with protoporphyrin IX in isolate sarcoma 180 cells
Enhancement of apoptosis by sonodynamic therapy with protoporphyrin IX in isolate sarcoma 180 cells
复制标题
DOI：
10.1089/cbr.2007.0436
复制
发表时间：
2008-04-01
影响因子：
3.4
通讯作者：
Wang, Bao Lu
中科院分区：
文献类型：
--
作者：
Wang, Xiao Bing;Liu, Quan Hong;Wang, Bao Lu
本研究旨在探讨原卟啉IX（protoporphyrin IX，PPIX）对超声诱导S180细胞凋亡的影响及其生物学机制。将S180细胞暴露于频率为2.2 MHz、声功率为3 W/cm（2）、含有120 μ M PPIX的超声波持续30秒。根据超声作用后不同孵育时间的形态学变化评价细胞凋亡。结果表明，照射可诱导S180肿瘤细胞凋亡，且随着照射时间的延长，凋亡率逐渐升高。我们的结果还表明Fas蛋白表达的变化与凋亡的发展相关。caspase-8和-3的活性明显上调凋亡，死亡底物（PARP）裂解的活性片段在一个时间依赖性的方式。这些结果表明，PPIX介导的声动力效应可能通过Fas介导的信号转导途径引发S180细胞凋亡而发挥其抗肿瘤作用。
This study was to investigate whether the apoptosis in isolate sarcoma 180 (S180) cells could be enhanced by ultrasound in the presence of protoporphyrin IX (PPIX) and also to evaluate the underlying biologic mechanism. S180 cells were exposed to ultrasound for 30 seconds' duration, at the frequency of 2.2 MHz and an acoustical power of 3 W/cm(2) with 120 mu M of PPIX. Cell apoptosis was evaluated based on the morphologic changes at different incubation times after sonication. Our results showed that the apoptosis in S180 tumor cells were induced by exposure, and the rate of apoptosis rose gradually with a longer incubation time. Our results also showed that changes in Fas protein expression were correlated with the development of apoptosis. The activities of caspase-8 and -3 were apparently upregulated by apoptosis, and the death substrate (PARP) was cleaved to active segments in a time-dependent manner. These results indicated that PPIX-mediated sonodynamic effect could exert its antitumor effect by triggering apoptosis in S180 cells through a Fas-mediated signal transduction pathway.