Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae
Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae
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DOI：
10.1016/j.cub.2007.07.062
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发表时间：
2007-09-18
期刊：
影响因子：
9.2
通讯作者：
Zwiebell, Laurence J.
中科院分区：
文献类型：
--
作者：
Lu, Tan;Qiu, Yu Tong;Zwiebell, Laurence J.
背景资料：许多种类的蚊子，包括主要的疟疾媒介冈比亚按蚊（Anopheles gambiae），利用二氧化碳（CO2）和1-辛烯-3-醇作为寻找宿主行为的嗅觉线索，这是其媒介能力的基础。然而，这样的嗅觉responses的分子和细胞基础仍然在很大程度上unknown.Results：在这里，我们使用分子和生理的方法，再加上系统的功能分析，以定义完整的嗅觉感觉地图的An。冈比亚上颚须，一种嗅觉附属物，介导这些化合物的检测。在这样做的时候，我们确定了三个嗅觉受体神经元（ORN），组织在刻板的黑社会内的上颌须头状栓感器人口。一个ORN是CO2-响应和其特征在于由三个受体，赋予CO2响应的共表达，而其他的ORN表达特征气味受体（AgORs），负责他们在体内的嗅觉responses.Conclusions：我们的研究结果描述了一个完整的和高度一致的地图的分子和细胞的嗅觉组件上的成年女性安的上颌须。大蚊子。这些结果也有助于理解An.冈比亚蚊子能够感知到可能被用来削弱其传播疟疾能力的嗅觉线索。
Background: Many species of mosquitoes, including the major malaria vector Anopheles gambiae, utilize carbon dioxide (CO2) and 1-octen-3-ol as olfactory cues in host-seeking behaviors that underlie their vectorial capacity. However, the molecular and cellular basis of such olfactory responses remains largely unknown.Results: Here, we use molecular and physiological approaches coupled with systematic functional analyses to define the complete olfactory sensory map of the An. gambiae maxillary palp, an olfactory appendage that mediates the detection of these compounds. In doing so, we identify three olfactory receptor neurons (ORNs) that are organized in stereotyped triads within the maxillary-palp capitate-peg-sensillum population. One ORN is CO2-responsive and characterized by the coexpression of three receptors that confer CO2 responses, whereas the other ORNs express characteristic odorant receptors (AgORs) that are responsible for their in vivo olfactory responses.Conclusions: Our results describe a complete and highly concordant map of both the molecular and cellular olfactory components on the maxillary palp of the adult female An. gmnbiae mosquito. These results also facilitate the understanding of how An. gambiae mosquitoes sense olfactory cues that might be exploited to compromise their ability to transmit malaria.