Comparative Tensile Properties and Collagen Patterns in Domestic Cat (Felis catus) and Dog (Canis lupus familiaris) Ovarian Cortical Tissues.
Comparative Tensile Properties and Collagen Patterns in Domestic Cat (Felis catus) and Dog (Canis lupus familiaris) Ovarian Cortical Tissues.
复制标题
DOI：
10.3390/bioengineering10111285
复制
发表时间：
2023-11-04
影响因子：
4.6
通讯作者：
Songsasen, Nucharin
中科院分区：
文献类型：
--
作者：
Nagashima, Jennifer B.;Zenilman, Shoshana;Raab, April;Aranda-Espinoza, Helim;Songsasen, Nucharin
近年来，卵巢细胞外环境和组织刚性对卵泡生存和发育的重要性得到了越来越多的关注。我们的实验室观察到家猫和狗卵巢皮质组织硬度的差异，这被认为是这两个物种体外培养反应差异的基础，其中猫卵巢组织在长时间孵育中表现出更高的存活率。本研究采用微管抽吸法比较猫和狗卵巢皮质组织的抗拉强度。通过微sirius红染色和TWOMBLI分析，定量观察胶原蛋白的结构，包括纤维的长度、厚度、排列、曲率、分支点和终点，以及整个组织腔隙和高密度基质（HDM）。最后，我们探索了MMP（- 1和- 9）和TIMP1补充在体外调节组织硬度、胶原结构和卵泡激活方面的潜力。在猫和狗的皮质组织之间，或者青春期前和青春期后的状态之间，没有观察到硬度的差异。与狗卵巢胶原蛋白相比，猫卵巢胶原蛋白的特点是分支点数量增加，纤维更细，HDM更低，并且暴露于MMP9的猫组织在体外表现出降低的杨氏模量。然而，MMP暴露对两个物种的卵泡发育都有轻微的影响。这项研究有助于我们对卵巢微环境的物理特性、胶原蛋白模式和体外卵泡发育之间的相互作用有更深入的了解。
The importance of the ovarian extracellular environment and tissue rigidity on follicle survival and development has gained attention in recent years. Our laboratory has anecdotally observed differences in the rigidity of domestic cat and dog ovarian cortical tissues, which have been postulated to underlie the differences in in vitro culture responses between the species, wherein cat ovarian tissues display higher survival in extended incubation. Here, the tensile strengths of cat and dog ovarian cortical tissues were compared via micropipette aspiration. The underlying collagen patterns, including fiber length, thickness, alignment, curvature, branch points and end points, and overall tissue lacunary and high-density matrix (HDM) were quantified via picrosirius red staining and TWOMBLI analysis. Finally, we explored the potential of MMP (−1 and −9) and TIMP1 supplementation in modulating tissue rigidity, collagen structure, and follicle activation in vitro. No differences in stiffness were observed between cat or dog cortical tissues, or pre- versus post-pubertal status. Cat ovarian collagen was characterized by an increased number of branch points, thinner fibers, and lower HDM compared with dog ovarian collagen, and cat tissues exposed to MMP9 in vitro displayed a reduced Young’s modulus. Yet, MMP exposure had a minor impact on follicle development in vitro in either species. This study contributes to our growing understanding of the interactions among the physical properties of the ovarian microenvironment, collagen patterns, and follicle development in vitro.
登录
查看更多内容
影响因子：
4.5
作者：
Lyons LA
通讯作者：
Lyons LA
DOI：
10.1186/s12958-023-01092-8
发表时间：
2023-05-19
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.3
作者：
Higgins, Debra F.;Kimura, Kuniko;Haase, Volker H.
通讯作者：
Haase, Volker H.
影响因子：
3.8
作者：
Aoki, T;Ohashi, T;Sato, M
通讯作者：
Sato, M
影响因子：
1.7
作者：
Fujihara, M.;Comizzoli, P.;Songsasen, N.
通讯作者：
Songsasen, N.