Age-related decline in VIP-positive parasympathetic nerve fibers in the human submacular choroid
Age-related decline in VIP-positive parasympathetic nerve fibers in the human submacular choroid
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DOI：
10.1167/iovs.06-0972
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发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
Reiner, Anton
中科院分区：
文献类型：
--
作者：
Jablonski, Monica M.;Iannaccone, Alessandro;Reiner, Anton
目的。黄斑脉络膜血流（ChBF）的年龄相关下降发生在人类。含有血管活性肠多肽（VIP）的血管舒张神经纤维支配脉络膜血管。目前的研究是为了研究人类黄斑下脉络膜中这些纤维与年龄相关的损失是否可能发生，从而导致chbf的下降。黄斑脉络膜穿孔从35名年龄在21岁至93岁之间的健康人类供体中收集。采用抗vip和过氧化物酶-抗过氧化物酶法对脉络膜样品进行免疫标记。vip阳性的神经纤维丰度在每次打孔中量化多达12个领域。分析了50个黄斑穿孔，并对眼睛的结果进行了平均。测量了相同视场的脉络膜血管直径（ChVD）。分析年龄与血管直径及vip阳性纤维丰度的关系。建立多元统计模型，校正性别、与组织标本相关的变量和潜在的程序变异源。完全调整的多变量模型显示，vip阳性纤维丰度（P = 0.0003，调整R-2 = 0.51）和ChVD （P < 0.0001，调整R-2 = 0.63）均与年龄相关，斜率分别为-0.45和-0.19。调整相同的变量，vip阳性纤维丰度与chvd有显著的直接相关性。结果表明，在无疾病的人类供体眼睛中，黄斑下脉络膜中vip阳性神经纤维和血管直径的显著年龄相关下降。这些发现表明，神经控制ChBF和血管直径的下降可能解释了临床观察到的ChBF及其适应性控制的下降。
PURPOSE. An age-related decline in macular choroidal blood flow ( ChBF) occurs in humans. Vasodilatory nerve fibers containing vasoactive intestinal polypeptide ( VIP) innervate choroidal blood vessels. The current study was conducted to examine the possibility that an age-related loss of these fibers might occur in the submacular choroid in humans, and thus contribute to a decline in ChBF.METHODS. Macular choroid punches were collected from 35 healthy human donors ranging from 21 to 93 years of age. Choroidal samples were immunolabeled using anti-VIP and the peroxidase-antiperoxidase method. VIP-positive nerve fiber abundance was quantified in up to 12 fields per punch. Fifty macular punches were analyzed, and results for eye pairs were averaged. Choroidal vessel diameter (ChVD) was measured for these same fields. The relationship between age and vessel diameter or VIP-positive fiber abundance was analyzed. Multivariate statistical models were generated correcting for gender, variables related to the tissue specimens, and potential procedural sources of variability.RESULTS. The fully adjusted multivariate models showed a significant age-related reduction in both the VIP-positive fiber abundance ( P = 0.0003, adjusted R-2 = 0.51) and ChVD ( P < 0.0001, adjusted R-2 = 0.63), with slopes of -0.45 and -0.19, respectively. Adjusting for the same variables, VIP-positive fiber abundance showed a significant direct correlation with ChVD.CONCLUSIONS. The results indicate a significant age-related decline in VIP-positive nerve fibers and vessel diameter in the submacular choroid in disease-free human donor eyes. These findings suggest that a decline in the neural control of ChBF and vessel diameter may explain the reductions in ChBF and its adaptive control observed clinically with aging.