Stimulation of DNA synthesis in senescent human cells following incubation with plasma membranes.
Stimulation of DNA synthesis in senescent human cells following incubation with plasma membranes.
复制标题
与质膜孵育后刺激衰老人类细胞中的 DNA 合成。
DOI：
10.1016/0014-4827(92)90090-u
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发表时间：
1992
影响因子：
3.7
通讯作者：
Cristofalo,VJ
中科院分区：
文献类型：
--
作者：
Frederich,KB;Phillips,PD;Cristofalo,VJ
DNA synthesis and mitosis were increased in mitogen-stimulated senescent WI-38 cells following incubation with plasma membranes prepared from young or senescent WI-38 cells, A431 cells, 3T3 cells, or NR6 cells. The percentage of [3H]thymidine-labeled nuclei in senescent cultures was two- to fivefold greater than that seen in controls in which cells were incubated in the absence of membranes or in the presence of boiled membranes. The effect was trypsin sensitive, suggesting that a protein moiety is necessary for stimulation of DNA synthesis. As the culture age increased, basal levels of DNA synthesis, as well as maximal stimulation of DNA synthesis following incubation with plasma membranes, decreased. These observations are consistent with the hypothesis that different subpopulations exist in senescing cultures and suggest a complex pattern of inhibitory and stimulatory regulation of cell proliferation.
