Impacts of maturation on the micromechanics of the meniscus extracellular matrix
Impacts of maturation on the micromechanics of the meniscus extracellular matrix
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DOI：
10.1016/j.jbiomech.2018.02.037
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发表时间：
2018-04-27
影响因子：
2.4
通讯作者：
Han, Lin
中科院分区：
文献类型：
--
作者：
Li, Qing;Wang, Chao;Han, Lin
为了阐明成熟过程在胶原纤维和纤维束的长度尺度上如何影响半月板细胞外基质（ECM）的结构和力学性能，我们通过原子力显微镜（AFM）-纳米压痕技术测试了胎儿和成年牛半月板的微观力学性能。对于环向纤维，我们检测到有效压痕模量（E - ind）随年龄显著增加。这种影响与成熟过程中胶原纤维直径的增加和排列的改善是一致的，并且在外侧区域更为明显，因为该区域的胶原纤维排列更整齐且更紧密。同时，成熟过程也显著增加了径向连接纤维的E - ind，但对完整表面或浅层的E - ind没有影响。这些结果为成熟过程对半月板ECM组装的影响提供了新的见解，并通过调节局部ECM结构和力学行为，为新的半月板修复策略的设计提供了可能。（C）2018爱思唯尔有限公司。保留所有权利。
To elucidate how maturation impacts the structure and mechanics of meniscus extracellular matrix (ECM) at the length scale of collagen fibrils and fibers, we tested the micromechanical properties of fetal and adult bovine menisci via atomic force microscopy (AFM)-nanoindentation. For circumferential fibers, we detected significant increase in the effective indentation modulus, E-ind, with age. Such impact is in agreement with the increase in collagen fibril diameter and alignment during maturation, and is more pronounced in the outer zone, where collagen fibrils are more aligned and packed. Meanwhile, maturation also markedly increases the E-ind of radial tie fibers, but not those of intact surface or superficial layer. These results provide new insights into the effect of maturation on the assembly of meniscus ECM, and enable the design of new meniscus repair strategies by modulating local ECM structure and mechanical behaviors. (C) 2018 Elsevier Ltd. All rights reserved.