Effect of daily lithium chloride administration on bone mass and strength in growing broiler chickens
Effect of daily lithium chloride administration on bone mass and strength in growing broiler chickens
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DOI：
10.3382/ps/peu079
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发表时间：
2015-02-01
期刊：
影响因子：
4.4
通讯作者：
Govoni, K. E.
中科院分区：
文献类型：
--
作者：
Harvey, B. M.;Eschbach, M.;Govoni, K. E.
目的是确定口服氯化锂补充对肉仔鸡骨强度和骨质量的影响。将96只肉鸡分配至2个处理组之一（氯化锂或对照组; n = 48/处理）。从1或3周龄开始，每天经口灌胃给予鸡氯化锂（20 mg/kg体重）或水。在6周龄时，将鸡安乐死，并收集骨骼和肌肉样品。24 h氯化锂（20 mg/kg体重）激发确定血清氯化锂在2 h内升高，并在24 h内清除系统，证明氯化锂的有效递送。处理没有影响体重（P >= 0.20）或采食量（P >= 0.81），表明氯化锂对肉鸡生长没有负面影响。为了测定骨强度，对从1周组的对照和氯化锂处理的鸟获得的股骨和胫骨进行3点弯曲。与对照组相比，氯化锂处理的鸡股骨刚度降低了22%（P = 0.02），而弹性模量没有相应的降低。在屈服或极限载荷以及相应的应力计算中没有观察到差异（P >= 0.26）。与对照组相比，氯化锂中钛的韧性没有改变（P = 0.11）。对对照组和氯化锂组的胫骨进行骨长度和micro-CT成像。骨长度、皮质骨或骨小梁体积、骨小梁厚度、数量或间距无差异（P >= 0.52）。氯化锂处理对胸肌颜色和脂质氧化无影响（P > 0.05）。总之，氯化锂处理对肉鸡的生长和肉品质没有负面影响。观察到用氯化锂处理的股骨的骨硬度降低，然而，与小鼠模型不同，本研究中使用的氯化锂剂量不会对肉鸡长骨产生合成代谢作用。
The objective was to determine the effects of oral lithium chloride supplementation on bone strength and mass in broiler chickens. Ninety-six broilers were assigned to 1 of 2 treatment groups (lithium chloride or control; n = 48/treatment). Beginning at 1 or 3 wk of age, chickens were administered lithium chloride (20 mg/kg body weight) or water daily by oral gavage. At 6 wk of age, chickens were euthanized and bone and muscle samples were collected. A 24 h lithium chloride (20 mg/kg body weight) challenge determined that serum lithium chloride increased within 2 h and cleared the system within 24 h, demonstrating the effective delivery of lithium chloride. Treatment did not influence body weight (P >= 0.20) or feed intake (P >= 0.81), demonstrating that lithium chloride did not negatively affect broiler growth. To determine bone strength, 3-point bending was performed on the femora and tibiae obtained from control and lithium chloride-treated birds in the 1 wk group. Lithium chloride-treated birds had a 22% reduction in stiffness compared with control in the femora (P = 0.02) without a corresponding reduction in elastic modulus. No differences were observed in yield or ultimate load and in the corresponding calculations of stresses (P >= 0.26). The toughness of tibiae was not altered in lithium chloride compared with control (P = 0.11). Bone length and micro-CT imaging were performed on the tibiae of control and lithium chloride groups. No differences (P >= 0.52) in bone length, cortical or trabecular bone volume, trabecular thickness, number, or spacing were observed. Lithium chloride treatment did not affect pectoralis muscle color or lipid oxidation (P > 0.05). In conclusion, lithium chloride treatment in broilers did not negatively affect growth or meat quality. A reduction in bone stiffness of the femur with lithium chloride treatment was observed, however unlike the mouse model, the dosages of lithium chloride used in the current study did not result in anabolic effects on broiler long bones.