Antiproteolytic effects of plasma from hibernating bears:: A new approach for muscle wasting therapy
Antiproteolytic effects of plasma from hibernating bears:: A new approach for muscle wasting therapy
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DOI：
10.1016/j.clnu.2007.07.003
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发表时间：
2007-10-01
影响因子：
6.3
通讯作者：
Argiles, Josep M.
中科院分区：
文献类型：
--
作者：
Fuster, Gernma;Busquets, Silvia;Argiles, Josep M.
背景与目的：在啮齿类动物和人类中，缺乏活动或饥饿会导致骨骼肌萎缩，包括肌肉细胞数量和大小的减少以及肌原纤维蛋白含量的减少。以前已经描述过，在越冬熊中，不活动不会引起骨骼肌细胞数量或大小的任何变化。考虑到所有这些因素，本研究的目的是测试冬眠熊血浆是否对孵育大鼠骨骼肌有抗A蛋白水解作用。方法：采用冬眠熊、非冬眠熊和对照熊血浆培养大鼠指长骨伸肌。之后，通过释放到培养基中的酪氨酸水平来评估蛋白水解率，并通过Northern blot检测不同蛋白水解系统的肌肉mRNA含量。结果：大鼠骨骼EDL肌肉在冬眠熊血浆中孵育，导致净蛋白水解率降低40%。这种蛋白水解的抑制伴随着两种溶酶体表达的减少。（组织蛋白酶13）和泛素依赖（泛素）蛋白水解系统。结论：结果表明，在冬眠期间，熊能够产生一种强大的蛋白水解抑制剂，释放到循环中，阻止与固定相关的肌肉萎缩。(c) 2007年爱思唯尔有限公司和欧洲临床营养与代谢学会。版权所有。
Background & aims: In rodents and humans, inactivity or starvation leads to atrophy of skeletal muscle including a decrease in the number and size of muscle cells and in the myofibrillar protein content. It has previously been described that in overwintering bears the inactivity does not provoke any toss of skeletal muscle cell number or size. Taking all these into account, the aim of this study is to test if hibernating bear plasma has any A antiproteolytic effect on incubated rat skeletal muscle.Methods: Rat skeletal extensor digitorum longus (EDL) muscles were incubated in the presence of hibernating, non-hibernating and control bear plasma. After that, proteoytic rate was evaluated as levels of tyrosine released to the medium and muscle mRNA content for different proteolytic systems were measured by Northern blot.Results: Rat skeletal EDL muscles incubation in the presence of hibernating bear plasma resulted in a 40% decrease of the net proteolytic rate. This inhibition of proteolysis was accompanied by decreases in the expression of both Lysosomal. (cathepsin 13) and ubiquitin-dependent (ubiquitin) proteolytic systems.Conclusions: The results suggest that during hibernation the bear is able to produce a powerful proteolytic inhibitor which is released to the circulation and blocks muscle wasting associated with immobilization. (c) 2007 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.