A COMPARATIVE BIOCHEMICAL, PHARMACOLOGICAL AND IMMUNOLOGICAL STUDY OF CLOSTRIDIUM-NOVYI ALPHA-TOXIN, CLOSTRIDIUM-DIFFICILE TOXIN-B AND C-SORDELLII LETHAL TOXIN
A COMPARATIVE BIOCHEMICAL, PHARMACOLOGICAL AND IMMUNOLOGICAL STUDY OF CLOSTRIDIUM-NOVYI ALPHA-TOXIN, CLOSTRIDIUM-DIFFICILE TOXIN-B AND C-SORDELLII LETHAL TOXIN
复制标题
DOI：
10.1016/0041-0101(91)90224-f
复制
发表时间：
1991-01-01
期刊：
影响因子：
2.8
通讯作者：
HABERMANN, E
中科院分区：
文献类型：
--
作者：
BETTE, P;OKSCHE, A;HABERMANN, E
三种梭状芽胞杆菌细胞毒素，即新城疫霉菌的α-毒素(Tox-α-nov)、艰难梭菌的B毒素(ToxB-dif)和索氏梭菌致死毒素(LT-SOR)由约200,000(Tox-α-Nov)、250,000(LT-SOR)和275,000(ToxB-dif)摩尔的单链组成。WTS。ToxB-dif和LT-SOR与兔多克隆抗体有交叉反应，而与Tox-α-NOV无交叉反应。对静脉注射的毒性。小鼠注射类似(LD50,100小时，50-200 ng/kg)，其特点是缓慢发展的液体丢失进入间质间隙。当注射到大鼠的爪子中时，毒素会导致延迟性的局部浮肿，持续数天。在体外，这三种毒素引起培养的猪肺动脉内皮细胞持续回缩。ToxB-dif和Tox-α-nov导致F-肌动蛋白在核周积聚，而LT-SOR则导致微丝结构的随机丧失。这些毒素抑制尿苷掺入内皮细胞或鸡胚胎细胞，而T84细胞的尿苷掺入增加约10倍。两种毒素都不是ADP核糖化肌动蛋白。这三种细胞毒素之间的相似性保证了它们被安排成一个共同的组，从而扰乱了微丝系统。
The three clostridial cytotoxins, i.e. alpha-toxin of C. novyi (Tox-alpha-nov), toxin B of C. difficile (ToxB-dif) and lethal toxin of C. sordellii (LT-sor) consist of single peptide chains of about 200,000 (Tox-alpha-nov), 250,000 (LT-sor) and 275,000 (ToxB-dif) mol. wts. ToxB-dif and LT-sor but not Tox-alpha-nov cross-reacted with rabbit polyclonal antibodies. Toxicity upon i.v. injection in mice was similar (LD50, 100 hr, 50-200 ng/kg) and was characterized by a slowly developing fluid loss into the interstitial space. When injected into the rat paw the toxins caused a delayed local edema lasting for days. In vitro the three toxins provoked a persistent retraction of endothelial cells cultured from pig pulmonary artery. ToxB-dif and Tox-alpha-nov triggered the accumulation of F-actin in the perinuclear region at the expense of the tight peripheral bands whereas LT-sor led to a random loss of microfilament structure. The toxins inhibited uridine incorporation into endothelial or chicken embryonic cells whereas T 84 cells responded by an about 10-fold increase of uridine incorporation. Neither toxin ADP-ribosylated actin. The similarities between the three cytotoxins warrant their arrangement into a common group which perturbs the microfilament system.