Cross-reactivity to human T-lymphotropic virus type III/lymphadenopathy-associated virus and molecular cloning of simian T-cell lymphotropic virus type III from African green monkeys.
Cross-reactivity to human T-lymphotropic virus type III/lymphadenopathy-associated virus and molecular cloning of simian T-cell lymphotropic virus type III from African green monkeys.
复制标题
与人类 T 淋巴细胞病毒 III 型/淋巴结病相关病毒的交叉反应以及来自非洲绿猴的猿 T 细胞嗜淋巴细胞病毒 III 型的分子克隆。
DOI：
10.1073/pnas.83.24.9754
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发表时间：
1986
影响因子：
11.1
通讯作者：
Mullins,JI
中科院分区：
文献类型：
--
作者：
Hirsch,V;Riedel,N;Kornfeld,H;Kanki,PJ;Essex,M;Mullins,JI
从包括非洲绿猴(STLV-IIIAGM)在内的多种灵长类动物中分离到的猴T淋巴细胞逆转录酶病毒(HTLV-III/LAV)具有与人类获得性免疫缺陷综合征的病原体相似的结构、抗原性和细胞病变特征。本报告描述了STLV-IIIAGM与HTLV-III/LAV之间的核酸交叉反应，STLV-IIIAGM基因组的分子克隆，以及其结构和与其他逆转录病毒的亲缘关系。来自感染单个动物病毒的细胞系的重叠克隆被发现覆盖了整个STLV-IIIAGM基因组，并显示出有限程度的限制性内切酶位点可变性。在该细胞系和其他STLV-IIIAGM感染细胞系的DNA中检测到特定的杂交片段。至少两个STLV-IIIAGM株中存在的一部分病毒DNA以未整合的病毒DNA形式存在，这是感染细胞病变逆转录病毒的特征。HTLV-III/LAV Poll和Gag基因与STLV-IIIAGM的交叉反应性最强，而STLV-IIIAGM与人T淋巴细胞嗜T细胞病毒I、II型(HTLV-I和II)、Visna病毒、牛白血病病毒或猫白血病病毒的分子克隆无交叉反应。
Simian T-lymphotropic retroviruses with structural, antigenic, and cytopathic features similar to the etiologic agent of human acquired immunodeficiency syndrome, human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV), have been isolated from a variety of primate species including African green monkeys (STLV-IIIAGM). This report describes nucleic acid cross-reactivity between STLV-IIIAGM and HTLV-III/LAV, molecular cloning of the STLV-IIIAGM genome, and evaluation of its structure and genetic relationship to other retroviruses. Overlapping clones from a cell line infected with virus from a single animal were found to encompass the entire STLV-IIIAGM genome and exhibit a limited degree of restriction-site variability. Specific hybridizing fragments were detected in DNA from this and other STLV-IIIAGM-infected cell lines. A fraction of viral DNA present in at least two STLV-IIIAGM lines persists as unintegrated viral DNA, a characteristic of infection with cytopathic retroviruses. Strongest cross-reactivity was detected between HTLV-III/LAV pol- and gag- genes and STLV-IIIAGM, whereas no cross-reactivity was detected between STLV-IIIAGM and molecular clones of human T-lymphotropic virus types I and II (HTLV-I and -II), visna virus, bovine leukemia virus, or feline leukemia virus.