Characterization of a newly established human chondrosarcoma cell line, CS-OKB
Characterization of a newly established human chondrosarcoma cell line, CS-OKB
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DOI：
10.1007/s004280050201
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发表时间：
1998-06-01
期刊：
影响因子：
--
通讯作者：
Hukuda, S
中科院分区：
文献类型：
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作者：
Chano, T;Okabe, H;Hukuda, S
一个克隆细胞系，CS-OKB，来自人软骨肉瘤，其特征在于细胞遗传学研究，免疫细胞化学染色，和逆转录聚合酶链反应（RT-PCR）。染色体异常特征的恶性软骨肿瘤进行了鉴定。CS-OKB细胞用抗II型胶原和抗硫酸角质素抗体强烈染色。RT-PCR结果表明，CS-OKB转录软骨特异性基因，如II型，X型前胶原，和聚集蛋白聚糖。这种人软骨肉瘤细胞系是稳定的，表达分化良好的软骨细胞特异性基因。它在无涂层的塑料培养皿中合成分化良好的软骨细胞特异性分子。CS-OKB可用于人软骨细胞的研究和人软骨肉瘤的特征。
A clonal cell line, CS-OKB, was derived from a human chondrosarcoma and characterized by cytogenetic study, immunocytochemical staining, and reverse transcriptase polymerase chain reaction (RT-PCR). Chromosomal abnormalities characteristic of malignant cartilaginous neoplasms were identified. CS-OKB cells were intensely stained with anti-type II collagen and anti-keratan sulphate antibodies. RT-PCR indicated that CS-OKB transcribes cartilage-specific genes such as type II, X procollagen, and aggrecan. This human chondrosarcoma cell line is stable and expresses well-differentiated chondrocyte-specific genes. It synthesizes well-differentiated chondrocyte-specific molecules in uncoated plastic dishes. CS-OKB may be useful for studies of human chondrocytes and in characterizing human chondrosarcomas.