Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis.
Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis.
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DOI：
10.1101/pdb.top107045
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发表时间：
2022-10
影响因子：
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通讯作者：
M. Guille;R. Grainger
中科院分区：
文献类型：
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作者：
M. Guille;R. Grainger
多年来，我们对生物系统的理解深受利用遗传方法的实验方法的影响。其中包括过度表达转基因或异位表达注射 RNA 的功能获得实验，以及敲除基因或敲除 RNA 的功能丧失实验。在这里，我们回顾了这些方法如何在非洲爪蟾中应用，并介绍了非洲爪蟾和热带非洲爪蟾基因操作的各种方案。
Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied in Xenopus frogs and introduce a variety of protocols for genetic manipulation of Xenopus laevis and Xenopus tropicalis.