Rapid high-throughput detection of diethylstilbestrol by using the arrayed langasite crystal microbalance combined with gold nanoparticles through competitive immunoassay
Rapid high-throughput detection of diethylstilbestrol by using the arrayed langasite crystal microbalance combined with gold nanoparticles through competitive immunoassay
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DOI：
10.1016/j.snb.2017.03.014
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发表时间：
2017-08
影响因子：
8.4
通讯作者：
Xiaoyan Liu;Yuan-dong Hu;X. Sheng;Yuan Peng;Jialei Bai;Quanjun Lv;Hongsheng Jia;H. Jiang;Zhixian Gao
中科院分区：
文献类型：
--
作者：
Xiaoyan Liu;Yuan-dong Hu;X. Sheng;Yuan Peng;Jialei Bai;Quanjun Lv;Hongsheng Jia;H. Jiang;Zhixian Gao
建立了一种基于金纳米颗粒（AuNPs）扩增的石英晶体微天平（QCM）快速检测己烯雌酚（DES）的方法，并将该方法应用于阵列硅酸镧晶体微天平（LCM）上，实现了DES的高通量检测。利用分子自组装技术在金电极表面制备了一种能特异识别己烯雌酚的敏感膜。金纳米颗粒（AuNPs）与抗DES抗体偶联以放大QCM的信号。DES的检测采用竞争免疫法。同时，为了制备阵列式LCM传感器，将五个金电极镀在单个硅酸镧镓晶体上。研究了阵列传感器的可靠性和效率。QCM传感器的线性检测范围为16 ~ 500 ng/mL，检测限为13 ng/mL。牛奶样品中己烯雌酚的回收率为98.0- 104.8%。通过使用结构类似物，QCM传感器平台被证明对DES的检测具有特异性。成功研制了阵列式LCM传感器，并实现了对DES的阵列式检测。
In this paper, a method for the rapid detection of diethylstilbestrol (DES) using quartz crystal microbalance (QCM) with gold nanoparticles (AuNPs) amplification was developed and this method was used on the arrayed langasite crystal microbalance (LCM) to realize the high-throughput detection of DES. A sensitive membrane which can recognize DES specifically was prepared on the surface of gold electrode through the molecular self-assembly technique. Gold nanoparticles (AuNPs) were coupled with anti-DES antibodies to amplify the signal of QCM. DES was detected through competitive immunoassay. At the same time, five gold electrodes were plated on a single langasite crystal in order to prepare an arrayed LCM sensor. The reliability and efficiency of the arrayed sensor were studied. The QCM sensor exhibits a linear detection range from 16 to 500 ng/mL and a detection limit of 13 ng/mL. The recovery rate of DES in the milk samples was 98.0–104.8%. By using structural analogs, the QCM sensor platform was demonstrated to be specific for the detection of DES. The arrayed LCM sensor was developed successfully and it could detect DES with arrayed method.