Determination of steroidal saponins in different organs of yam (Dioscorea pseudojaponica Yamamoto)
Determination of steroidal saponins in different organs of yam (Dioscorea pseudojaponica Yamamoto)
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DOI：
10.1016/j.foodchem.2007.11.041
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发表时间：
2008-06-01
期刊：
影响因子：
8.8
通讯作者：
Yang, Deng-Jye
中科院分区：
文献类型：
--
作者：
Lin, Jau-Tien;Yang, Deng-Jye
山药（番薯属）是多年生蔓生根茎植物。甾体皂苷、呋甾烷醇和螺甾烷醇糖苷是山药中具有显著功能的化合物。本研究建立了C18固相萃取分离纯化山药皂苷的方法。同时测定了山药各器官中皂甙的含量。结果表明，当薯蓣皂苷质量分别为0.20、0.50和1.00 mg时，该方法提取的薯蓣皂苷的回收率在99.48 - 100.08%之间。该提取方法可有效降低薯蓣皂苷提取物中杂质对HPLC分析的干扰。山药不同器官中皂苷的加标回收率分别为：薯肉98.3499.92%，薯皮95.98-98.89%，根茎97.89-99.44%，叶93.82-98.01%，藤93.87-97.65%。山药块茎皮层中皂苷含量最高（582.53 μ g/gdw），是块茎果肉中皂苷含量（227.86 μ g/gdw）的2.55倍。山药根茎、叶和藤中皂苷的含量分别为29.39、24.41和23.96 μ g/gdw。(C)2007爱思唯尔有限公司保留所有权利。
Yams (Dioscorea spp.) are perennial trailing rhizome plants. Steroidal saponins, furostanol and spirostanol glycosides are the marked functional compounds in yams. In this investigation, a C18 solid phase extraction method was developed for yam saponins purification. The contents of saponins in various organs of yam (Dioscorea pseudojaponica Yamamoto) were also determined. Results showed that the recoveries of yam saponins extracted by the developed method were about 99.48-100.08% when the saponins (each saponin weighed 0.20, 0.50 and 1.00 mg) passing through the C18 cartridge. The extractive method could efficiently reduce the interferences from impurities in yam saponin extracts prior to HPLC analysis. The recoveries of added saponins in different yam organs were 98.3499.92% for tuber flesh, 95.98-98.89% for tuber cortex, 97.89-99.44% for rhizophor, 93.82-98.01% for leaf and 93.87-97.65% for vine, respectively. The yam tuber cortex had the highest amount of saponins (582.53 mu g/g dw), which was higher than that existed in the tuber flesh (227.86 mu g/g dw) about 2.55 times. The contents of saponins in the rhizophor, leaf and vine of yam were 29.39, 24.41 and 23.96 mu g/g dw, respectively. (C) 2007 Elsevier Ltd. All rights reserved.