Methylmercury alters proliferation, migration, and antioxidant capacity in human HTR8/SV-neo trophoblast cells.
Methylmercury alters proliferation, migration, and antioxidant capacity in human HTR8/SV-neo trophoblast cells.
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DOI：
10.1016/j.reprotox.2018.03.008
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Nowak RA
中科院分区：
文献类型：
--
作者：
Tucker EK;Nowak RA
甲基汞是一种强效神经毒素，能够通过胎盘，但其对胎盘本身的影响尚未阐明。使用永生化的人滋养层细胞系，HTR 8/SV-neo，我们评估了甲基汞的体外毒性。我们发现，1 μg/mL甲基汞降低了生存力，增殖和迁移;它对抗氧化基因的影响与神经细胞中观察到的相似。而甲基汞则导致超氧化物歧化酶1的表达降低和表面活性蛋白D的表达增加。0.01或0.1 μg/mL甲基汞处理的HTR细胞迁移率沿着粘附基因钙粘蛋白3表达的降低，表明低剂量甲基汞促进HTR细胞的迁移。我们的研究结果表明，滋养层细胞的反应不同的甲基汞相对于神经细胞系，因此，调查甲基汞在胎盘细胞的毒性是必要的，以了解这种重金属对胎盘的影响。
Methylmercury, a potent neurotoxin, is able to pass through the placenta, but its effects on the placenta itself have not been elucidated. Using an immortalized human trophoblast cell line, HTR8/SV-neo, we assessed the in vitro toxicity of methylmercury. We found that 1 μg/mL methylmercury decreased viability, proliferation, and migration; and it had effects on antioxidant genes similar to those seen in neural cells. However, methylmercury led to decreased expression of superoxide dismutase 1 and increased expression of surfactant protein D. HTR cells treated 0.01 or 0.1 μg/mL methylmercury had increased migration rates along with decreased expression of an adhesion gene, cadherin 3, suggesting that low doses of methylmercury promote migration in HTR cells. Our results indicate that trophoblast cells react differently to methylmercury relative to neural cell lines, and thus investigation of methylmercury toxicity in placental cells is needed to understand the effects of this heavy metal on the placenta.
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影响因子：
11.4
作者：
Gottfredsen, Randi H.;Larsen, Ulrike G.;Enghild, Jan J.;Petersen, Steen V.
通讯作者：
Petersen, Steen V.
影响因子：
2.9
作者：
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通讯作者：
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DOI：
10.1073/pnas.1120747109
发表时间：
2012-03-27
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
9.8
作者：
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通讯作者：
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