Noninvasive Intraocular pressure measurements in mice by pneumotonometry
Noninvasive Intraocular pressure measurements in mice by pneumotonometry
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DOI：
10.1167/iovs.04-1188
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发表时间：
2005-09-01
影响因子：
4.4
通讯作者：
Civan, MM
中科院分区：
文献类型：
--
作者：
Avila, MY;Múnera, A;Civan, MM
目的.目的：建立一种可靠、无创、连续、简便的小鼠眼压测量系统。采用气压眼压计测量小鼠IOP。将测量结果与采用伺服零位微量移液管系统（SNMS）和直接前房插管获得的测量结果进行比较。通过麻醉小鼠的心前区脉搏、EKG或尾脉监测心率。对甘露醇的特征性眼部肿胀反应进行了评估，作为该方法的额外验证。使用肺压测定法获得的IOP测量值与SNMS或直接插管测量值密切相关。IOP振荡与心率同步，它们之间的相干峰类似于2 Hz，等于脉冲频率。15分钟后，高渗甘露醇使IOP从13.7 ± 0.9 mm Hg降低7.7 ± 0.7 mm Hg。肺眼压计是一种可靠的和非侵入性的方法，用于测量小鼠的IOP，并可能允许IOP的血流动力学因素进行比较。该系统比以前报道的测量小鼠IOP的方法更简单，更适用于青光眼研究。
PURPOSE. To develop a reliable, noninvasive, continuous, and easily implemented system for measuring intraocular pressure ( IOP) in mice.METHODS. Pneumotonometry was adapted for measurement of mouse IOP. Measurements were compared with those obtained with the servo- null micropipette system ( SNMS) and with direct anterior chamber cannulation. Heart rate was monitored by the precordial pulse, EKG, or tail pulse in anesthetized mice. The characteristic ocular hypotensive response to mannitol was assessed as an additional validation of the method.RESULTS. Measurements of IOP obtained using pneumotonometry agreed closely with values measured by SNMS or by direct cannulation. IOP oscillations were synchronous with the heart rate, with a coherence peak between them of similar to 2 Hz, equal to the pulse frequency. Hypertonic mannitol reduced IOP from 13.7 +/- 0.9 mm Hg by 7.7 +/- 0.7 mm Hg after 15 minutes.CONCLUSIONS. Pneumotonometry is a reliable and noninvasive method for the measurement of IOP in mice and may permit comparisons of IOP to hemodynamic factors. This system is simpler and more adaptable for glaucoma research than previously reported methodologies for measuring IOP in mice.