Specific cytokine patterns of pulmonary tuberculosis in Central Africa
Specific cytokine patterns of pulmonary tuberculosis in Central Africa
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DOI：
10.1016/j.clim.2010.09.005
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发表时间：
2011-01-01
影响因子：
8.6
通讯作者：
Winkler, Stefan
中科院分区：
文献类型：
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作者：
Nemeth, Johannes;Winkler, Heide-Maria;Winkler, Stefan
不同的细胞因子被认为参与了肺结核（TB）的发病机制。结核分枝杆菌（MTB）特异性CIA的频率。在加蓬的HIV阴性肺结核（TB）患者（n=30）和健康对照（n=23）中评估了CD 8（+）T细胞、CD 4（+）CD 25（+）叉头盒蛋白（FoxP）3（+）T细胞、白细胞介素（IL）-6、干扰素（IFN）-γ、肿瘤坏死因子（TNF）-α、转化生长因子（TGF）-β和IL-10。用纯化的蛋白衍生物（PPD）和早期分泌性抗原靶标-6（ESAT-6）刺激外周血单个核细胞（PBMC）。在患者中，可检测到显著的促炎细胞因子应答，其中IL-6和TNF-α水平高度显著升高，并伴有TGF-β升高。患者和健康个体之间IFN-γ反应的差异不如预期的明显。FoxP 3表达在各组之间没有差异。一种独特的细胞因子模式与中非患者的活动性肺结核相关。(C)2010年爱思唯尔公司All rights reserved.
Different cytokines have been suggested to be involved in the pathogenesis of pulmonary tuberculosis (TB). The frequencies of Mycobacterium tuberculosis (MTB) specific CIA. and CD8(+) T cells, CD4(+)CD25(+) Forkhead Box Protein (FoxP)3(+) T cells, interleukin (IL)-6, interferon (IFN)-gamma, Tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta and IL-10 were assessed in HIV-negative, pulmonary tuberculosis (TB) patients (n=30) and in healthy controls (n=23) in Gabon. Peripheral blood mononuclear cells (PBMC) were stimulated with purified protein derivative (PPD) and early secretory antigenic target-6 (ESAT-6). In patients, a pronounced pro-inflammatory cytokine response with highly significant increased levels of IL-6 and TNF-alpha accompanied by increased TGF-beta was detectable. Differences in IFN-gamma responses between patients and healthy individuals were less pronounced than expected. FoxP3 expression did not differ between groups. A distinct cytokine pattern is associated with active pulmonary TB in patients from Central Africa. (C) 2010 Elsevier Inc. All rights reserved.