Genomic insights into the Ixodes scapularis tick vector of Lyme disease.
Genomic insights into the Ixodes scapularis tick vector of Lyme disease.
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DOI：
10.1038/ncomms10507
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发表时间：
2016-02-09
影响因子：
16.6
通讯作者：
Hill CA
中科院分区：
文献类型：
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作者：
Gulia-Nuss M;Nuss AB;Meyer JM;Sonenshine DE;Roe RM;Waterhouse RM;Sattelle DB;de la Fuente J;Ribeiro JM;Megy K;Thimmapuram J;Miller JR;Walenz BP;Koren S;Hostetler JB;Thiagarajan M;Joardar VS;Hannick LI;Bidwell S;Hammond MP;Young S;Zeng Q;Abrudan JL;Almeida FC;Ayllón N;Bhide K;Bissinger BW;Bonzon-Kulichenko E;Buckingham SD;Caffrey DR;Caimano MJ;Croset V;Driscoll T;Gilbert D;Gillespie JJ;Giraldo-Calderón GI;Grabowski JM;Jiang D;Khalil SMS;Kim D;Kocan KM;Koči J;Kuhn RJ;Kurtti TJ;Lees K;Lang EG;Kennedy RC;Kwon H;Perera R;Qi Y;Radolf JD;Sakamoto JM;Sánchez-Gracia A;Severo MS;Silverman N;Šimo L;Tojo M;Tornador C;Van Zee JP;Vázquez J;Vieira FG;Villar M;Wespiser AR;Yang Y;Zhu J;Arensburger P;Pietrantonio PV;Barker SC;Shao R;Zdobnov EM;Hauser F;Grimmelikhuijzen CJP;Park Y;Rozas J;Benton R;Pedra JHF;Nelson DR;Unger MF;Tubio JMC;Tu Z;Robertson HM;Shumway M;Sutton G;Wortman JR;Lawson D;Wikel SK;Nene VM;Fraser CM;Collins FH;Birren B;Nelson KE;Caler E;Hill CA
蜱比其他任何节肢动物都能将更多的病原体传播给人类和动物。我们描述了2.1 Gbp的核基因组的蜱，肩突硬蜱（说），其中载体的病原体，导致莱姆病，人类粒细胞无形体病，巴贝虫病和其他疾病。大的基因组反映了重复DNA的积累，逆转录转座子的新谱系，以及类似于古代后生动物而不是泛甲壳动物的基因结构模式。对占基因组57%的支架进行注释，揭示了20，486个蛋白质编码基因和与蜱虫-宿主相互作用相关的基因家族的扩展。我们报告的见解，从基因组分析到寄生虫的过程中独特的蜱，包括主机的'questing'，长期喂养，角质层合成，血粉浓度，血红蛋白消化，血红素解毒，卵黄发生和延长脱宿主生存的新方法。我们确定了与人粒细胞无形体病，一种新出现的疾病，和脑炎引起的Langat病毒的代理相关的蛋白质，以及与莱姆病代理的生活史特征和传播相关的人口结构。 蜱传播大量病原体，导致人类疾病。在这里，作者对蜱的肩突硬蜱的基因组进行了测序，并发现了与蜱特有的寄生过程和蜱-宿主相互作用相关的基因的扩增。
Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ∼57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick–host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host ‘questing', prolonged feeding, cuticle synthesis, blood meal concentration, novel methods of haemoglobin digestion, haem detoxification, vitellogenesis and prolonged off-host survival. We identify proteins associated with the agent of human granulocytic anaplasmosis, an emerging disease, and the encephalitis-causing Langat virus, and a population structure correlated to life-history traits and transmission of the Lyme disease agent. Ticks transmit a large number of pathogens that cause human diseases. Here, the authors sequence the genome of the tick Ixodes scapularis and uncover expansion of genes associated with parasitic processes unique to ticks and tick-host interactions.