The effects of three bioreductive drugs (mitomycin C, RSU-1069 and SR4233) on cell lines selected for their sensitivity to mitomycin C or ionising radiation.
The effects of three bioreductive drugs (mitomycin C, RSU-1069 and SR4233) on cell lines selected for their sensitivity to mitomycin C or ionising radiation.
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三种生物还原药物（丝裂霉素 C、RSU-1069 和 SR4233）对因对丝裂霉素 C 或电离辐射敏感而选择的细胞系的影响。
DOI：
10.1038/bjc.1990.162
复制
发表时间：
1990
影响因子：
8.8
通讯作者：
Adams,GE
中科院分区：
文献类型：
--
作者：
Keohane,A;Godden,J;Stratford,IJ;Adams,GE
我们研究了在有氧和低氧条件下，一些细胞系对三种不同类型的生物还原药物的交叉敏感性。所用的细胞系根据其对DNA损伤剂的敏感性进行选择，并分为两组。一组是源自CHO-K1细胞的MMC细胞（Robson等人，1985），显示了对空气中丝裂霉素C的一系列敏感性。第二组，从V79中国仓鼠成纤维细胞克隆irs细胞（Jones等人，1987），并表现出对电离辐射的敏感性。在有氧和低氧条件下评估两组细胞对丝裂霉素C（MMC）、RSU-1069和SR 4233的敏感性。MMC对CHO-K1或MMC敏感细胞系（MMC-2和MMC-3）的有氧或缺氧毒性无差异。然而，MMC耐药细胞系（MMCr）在缺氧条件下比有氧条件下敏感10倍。这表明与其他MMC细胞相比，MMCr细胞缺乏或缺乏负责在有氧条件下激活MMC的酶。相比之下，对于用RSU-1069和SR 4233处理的所有CHO细胞，观察到的毒性差异在3至30之间。用RSU-1069和SR 4233处理V79和irs细胞也导致对缺氧细胞的选择性毒性。对于V79细胞，观察到50和100之间的差异毒性。对于RSU-1069和SR 4233，低氧毒性在V79和irs细胞中相似，但在空气中，辐射敏感细胞的敏感性比野生型细胞高10倍。
We have investigated the cross-sensitivity of a number of cell lines to three different classes of bioreductive drugs under both aerobic and hypoxic conditions. The cell lines used were selected for their sensitivity to DNA-damaging agents and fall into two groups. One group, MMC cells derived from CHO-K1 cells (Robson et al., 1985), show a range of sensitivities to mitomycin C in air. The second group, irs cells were cloned from V79 Chinese hamster fibroblasts (Jones et al., 1987) and exhibit sensitivity to ionising radiation. The sensitivity of both groups of cells to mitomycin C (MMC), RSU-1069 and SR4233 was assessed under aerobic and hypoxic conditions. No difference in aerobic or hypoxic toxicity of MMC was observed for CHO-K1 or MMC sensitive cell lines (MMC-2 and MMC-3). However, the MMC-resistant cell line (MMCr) was 10 times more sensitive under hypoxic than aerobic conditions. This suggests that MMCr cells lack or are deficient in the enzymes responsible for activating MMC under aerobic conditions compared to other MMC cells. In contrast, differential toxicities of between 3 and 30 have been observed for all CHO cells treated with RSU-1069 and SR4233. Treatment of V79 and irs cells with RSU-1069 and SR4233 also resulted in selective toxicity towards hypoxic cells. Differential toxicities between 50 and 100 were observed for V79 cells. For both RSU-1069 and SR4233, the hypoxic toxicities were similar in V79 and irs cells but in air, the radiation sensitive cells were up to 10 times more sensitive than wild type cells.
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DOI：
10.1016/0006-291x(75)90163-1
发表时间：
1975-01-01
影响因子：
3.1
作者：
MASON, RP;HOLTZMAN, JL
通讯作者：
HOLTZMAN, JL
NADPH：细胞色素 c 还原酶和 DT-心肌黄酶在丝裂霉素 C1 生物转化中的作用。
影响因子：
11.2
作者：
Keyes,SR;Fracasso,PM;Heimbrook,DC;Rockwell,S;Sligar,SG;Sartorelli,AC
通讯作者：
Sartorelli,AC
DOI：
10.1016/0360-3016(89)90900-0
发表时间：
1989-04-01
影响因子：
7
作者：
WALTON, MI;WOLF, CR;WORKMAN, P
通讯作者：
WORKMAN, P
RSU 1069，一种含有氮丙啶基团的硝基咪唑。
影响因子：
5.8
作者：
I. Stratford;P. O'Neill;P. Sheldon;A. Silver;J. Walling;G. Adams
通讯作者：
G. Adams
辐射敏感 irs 突变体中的 DNA 断裂修复、DNA 合成的辐射抗性和喜树碱敏感性：与共济失调毛细血管扩张细胞的比较。
DOI：
10.1016/0921-8777(90)90057-c
发表时间：
1990
期刊：
Mutation research
影响因子：
--
作者：
J. Thacker;A. Ganesh
通讯作者：
A. Ganesh