Selective uptake of radiolabeled annexin V on acute porcine left atrial thrombi.
Selective uptake of radiolabeled annexin V on acute porcine left atrial thrombi.
复制标题
急性猪左心房血栓上放射性标记的膜联蛋白 V 的选择性摄取。
DOI：
10.1161/01.cir.92.10.3113
复制
发表时间：
1995
期刊：
影响因子：
37.8
通讯作者：
Tait,JF
中科院分区：
文献类型：
--
作者：
Stratton,JR;Dewhurst,TA;Kasina,S;Reno,JM;Cerqueira,MD;Baskin,DG;Tait,JF
背景膜联蛋白V是一种磷脂结合蛋白，在体外可与活化的血小板结合。我们试图确定潜在的这种代理成像心内血栓在swines.Methods和ResultsThe左心房血栓形成挤压伤。在使用静脉注射125 I标记的人膜联蛋白V的初始非成像实验中，使用切除标本的孔计数，整个血栓的平均血栓/全血比为13.4±4.8（n=8）。用静脉注射99 mTc标记的人膜联蛋白V，通过孔计数的左心房血栓/血液比率相似（整个血栓为14.2±10.6，峰部分为26.2±14.9）（n=12）。对照蛋白125 I-卵清蛋白的比值仅为1.0±0.2.99mTc断层显像在所有实验中均为阳性（n=10）或可疑（n=2），而在10个对照中无左房血栓为阴性。通过断层图像的感兴趣区域分析，血栓动物2小时的平均左心耳/血液比为3.90±1.12，而闭胸对照组为0.84±0.10，开胸对照组为1.01±0.23（P<0.001）。结合一种新的血栓检测剂，膜联蛋白V，与99 mTc标记，可能允许在体内成像的血栓在人类。
BackgroundAnnexin V is a human phospholipid binding protein that binds to activated platelets in vitro. We sought to determine the potential of this agent for imaging intracardiac thrombi in swine.Methods and ResultsLeft atrial thrombi were formed by crush injury. In initial nonimaging experiments using intravenous125I-labeled human annexin V, the mean thrombus/whole blood ratio was 13.4±4.8 for the entire thrombus using well counting of resected specimens (n=8). Using intravenously injected99mTc-labeled human annexin V, the left atrial thrombus/blood ratio by well counting was similar (14.2±10.6 for the entire thrombus and 26.2±14.9 for the peak section) (n=12). The ratio for a control protein,125I-ovalbumin, was only 1.0±0.2.99mTc tomographic imaging was positive (n=10) or equivocal (n=2) in all experiments with but negative in 10 controls without left atrial thrombi. By region-of-interest analysis of the tomographic images, the mean left atrial appendage/blood ratio at 2 hours in animals with a thrombus was 3.90±1.12 compared with 0.84±0.10 in closed chest controls and 1.01±0.23 in open chest controls (P<.001).ConclusionsWe conclude that99mTc-labeled human annexin V detects acute left atrial thrombi in vivo in swine. The combination of a new thrombus detection agent, annexin V, with a99mTc label may allow in vivo imaging of thrombi in humans.