Systemic distribution and elimination of plain and with Cy3.5 functionalized poly(vinyl alcohol) coated superparamagnetic maghemite nanoparticles after intraarticular injection in sheep in vivo
Systemic distribution and elimination of plain and with Cy3.5 functionalized poly(vinyl alcohol) coated superparamagnetic maghemite nanoparticles after intraarticular injection in sheep in vivo
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DOI：
10.1166/jnn.2006.482
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发表时间：
2006-09-01
影响因子：
--
通讯作者：
von Rechenberg, Brigitte
中科院分区：
文献类型：
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作者：
Hellstern, Daniel;Schulze, Katia;von Rechenberg, Brigitte
评价了PVA包衣和荧光染料(Cy3.5)功能化乙烯醇/乙烯胺共聚物包衣的超顺磁性氧化铁纳米粒(SPION)在绵羊关节内注射后的全身分布和消除情况。分别在注射后3、24、72、120小时用光学显微镜、荧光显微镜和共聚焦显微镜进行观察。未见Spion对采集的组织有病理影响。注射Spion后，肾脏、淋巴结和脾中铁含量显著增加。在肝脏、尿液和胆汁中未检测到颗粒。没有阳性的荧光信号可归因于SPION遍及各器官。我们的结果表明，Spion的铁成分在肾脏近端小管系统重吸收后可能被并入生理性铁代谢，而Cy3.5的浓度水平太低，无法在全身检测到。
PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy. No pathologic influence of SPION on the tissue harvested could be seen. A significantly increased iron content could be identified in the kidneys, lymph nodes, and spleen after injection of SPION. No particles were detected in the liver, the urinary, and the gall bladder. No positive fluorescent signal could be attributed to SPION throughout the organs. Our results indicated that the iron component of the SPION is possible to be incorporated into the physiologic iron metabolism after reabsorption in the proximal tubule system of the kidney and that concentration levels of Cy3.5 are too low to be detected throughout the body.