COMPARISON OF TWO FORMS OF LOW CALCIUMREQUIRING PROTEASE
COMPARISON OF TWO FORMS OF LOW CALCIUMREQUIRING PROTEASE
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两种低钙需求蛋白酶的比较
DOI：
10.2220/biomedres.3.699
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发表时间：
1982
影响因子：
1.2
通讯作者：
Koichi Suzuki
中科院分区：
文献类型：
--
作者：
S. Kubota;Koichi Suzuki
从兔骨骼肌中艾德了两种形式的钙激活中性蛋白酶（CANP）。一种是低钙需求型（CANP I），另一种是高钙需求型（CANP II）。以前我们报道过CANP II通过自溶转化为低钙需要形式（uCANP）。我们将uCANP与CANP I进行比较以确定它们的身份。它们在对钙离子的敏感性、与蛋白酶抑制剂的反应性和pH活性特性方面相似，但它们的分子量和稳定性不同。我们得出结论，CANP I和uCANP是不同形式的蛋白酶，其在低浓度的钙离子下是活性的。CANP来源于猪骨骼肌、兔骨骼肌、人骨骼肌、鸡骨骼肌功能细胞蛋白质，它能去除W 'tr0 al.代顿尔艾的Z线。活性水平的钙离子高钙需要型CANP（CANP低钙需要型CANP
Two forms of a calcium activated neutral protease (CANP) were purified from rabbit skeletal muscle to homogeneity. One is a low calcium-requiring form (CANP I) and the other is a high calcium-requiring form (CANP II). Previously we reported that CANP II was converted to the low calcium-requiring form (,uCANP) by autolysis. We compared ,uCANP with CANP I to determine their identity. They were similar with respect to sensitivity to calcium ions, reaction with protease inhibitors and pH activity profiles, but their molecular weights and stabilities were different. We conclude that CANP I and ,uCANP are different forms of a protease which is active at low concentrations of calcium ions. CANPs from porcine skeletal rabbit skeletal muscle human skeletal chicken skeletal muscle function cell proteins it removes Z-line in w'tr0 al. Dayton er ai. active levels of calcium ions high calcium-requiring CANP (CANP low calcium-requiring CANP