Association between in vivo fluorine 18-labeled flutemetamol amyloid positron emission tomography imaging and in vivo cerebral cortical histopathology.
Association between in vivo fluorine 18-labeled flutemetamol amyloid positron emission tomography imaging and in vivo cerebral cortical histopathology.
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DOI：
10.1001/archneurol.2011.153
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发表时间：
2011-11
影响因子：
--
通讯作者：
Arnold SE
中科院分区：
文献类型：
--
作者：
Wolk DA;Grachev ID;Buckley C;Kazi H;Grady MS;Trojanowski JQ;Hamilton RH;Sherwin P;McLain R;Arnold SE
确定[18F]-氟代米特脑摄取的体内定量估计值与既往活检患者淀粉样蛋白水平的免疫组织化学估计值的对应性。对既往皮质活检染色检查是否存在淀粉样斑块的患者进行[18F]-氟代戊二酸正电子发射断层扫描（PET）结果的横断面研究。大学医院。招募了7名先前在脑室-腹膜（VP）放置部位进行过右侧额叶皮质活检的患者，用于假定的正常压力脑积水（NPH）。入选标准包括足够的活检以检测和定量Aβ病理学和年龄大于50岁。所有患者均接受了[18F]-Flutemetalone PET扫描。在活检部位的对侧位置进行[18 F]-flutemetetaline摄取的定量测量（SUVR -平均靶皮质活性除以小脑参考区域的活性的比值），并与基于免疫组织化学和组织学染色的淀粉样蛋白负荷估计值进行比较。[18F]-flutemetalone PET扫描的视觉读数（三名盲读者，多数规则）和组织学之间完全一致。包括活检时间作为协变量的回归模型表明，[18 F]-氟代蛋氨酸摄取与淀粉样蛋白（NAB 228）单克隆抗体测量的淀粉样蛋白面积百分比之间存在显著相关性（p=0.011）。用淀粉样蛋白特异性单克隆抗体4G 8和硫磺素S也发现了类似的结果。这些数据首次证明了[18F]-flutemetaldehyde PET成像与组织病理学的一致性，支持其检测淀粉样蛋白的灵敏度以及在阿尔茨海默病研究和检测中的潜在用途。
To determine the correspondence of in vivo quantitative estimates of brain uptake of [18F]-flutemetamol with immunohistochemical estimates of amyloid levels in previously biopsied patients. Cross-sectional study of [18F]-flutemetamol positron emission tomography (PET) findings in patients with prior cortical biopsy stained for the presence or absence of amyloid plaques. University Hospital. Seven patients who previously had a prior right frontal cortical biopsy obtained at the site of ventriculo-peritoneal (VP) placement for presumed Normal Pressure Hydrocephalus (NPH) were recruited. Inclusion criteria included an adequate biopsy for detection and quantification of Aβ pathology and age greater than 50 years. All patients underwent an [18F]-flutemetamol PET scan. Quantitative measures of [18F]-flutemetamol uptake (SUVR – ratio of mean target cortex activity divided by that in a cerabellar reference region) were made at a location contralateral to the biopsy site and compared to estimates of amyloid load based on immunohistochemical and histological staining. There was complete agreement between visual reads of [18F]-flutemetamol PET scans (three blinded readers with majority rule) and histology. A regression model, including time from biopsy as a covariate, demonstrated a significant relationship (p=0.011) between [18F]-flutemetamol uptake and percent area of amyloid measured by a monoclonal antibody raised against amyloid (NAB228). Similar results were found with the amyloid specific monoclonal antibody 4G8 and Thioflavin S. These data are the first to demonstrate the concordance of [18F]-flutemetamol PET imaging with histopathology, supporting its sensitivity to detect amyloid and potential use in the study and detection of Alzheimer’s Disease.