Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration
Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration
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DOI：
10.1016/s0012-1606(03)00129-5
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发表时间：
2003-06-15
影响因子：
2.7
通讯作者：
Keating, MT
中科院分区：
文献类型：
--
作者：
Nechiporuk, A;Poss, KD;Keating, MT
在这里，我们使用斑马鱼的经典遗传学来鉴定尾鳍再生中的温度敏感突变体。形态学、组织学和分子分析显示，其中一株emmental （emm）未能形成功能性再生胚。在再生生长过程中，热处理对emm功能的抑制迅速阻断了再生。这种阻滞与近端囊胚增殖减少和非增殖的远端囊胚区扩张有关。定位克隆表明，emm表型是由酵母sle1同源基因突变引起的，sle1是一种参与蛋白质运输的基因产物。在新形成的胚芽和再生生长过程中，Sly1表达上调。因此，在鱼鳍再生的两个阶段中，sle1对胚芽组织和增殖至关重要。(C) 2003 Elsevier Science（美国）版权所有。
Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, and molecular analyses revealed that one of these strains, emmental (emm), failed to form a functional regeneration blastema. Inhibition of emm function by heat treatment during regenerative outgrowth rapidly blocked regeneration. This block was associated with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal blastemal zone. Positional cloning revealed that the emm phenotype is caused by a mutation in the orthologue of yeast sly1, a gene product involved in protein trafficking. sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth. Thus, sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration. (C) 2003 Elsevier Science (USA). All rights reserved.