Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk, in the bio-control of Listeria monocytogenes in fresh whey cheese
Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk, in the bio-control of Listeria monocytogenes in fresh whey cheese
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DOI：
10.1016/j.idairyj.2017.04.008
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发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Papademas, Photis
中科院分区：
文献类型：
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作者：
Aspri, Maria;O'Connor, Paula M.;Papademas, Photis
共79个细菌菌株，以前从驴奶中分离，筛选其对几个腐败和食源性致病菌的抗菌活性。其中3株屎肠球菌对单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌具有抗菌活性。质谱分析表明，本研究中使用的所有肠球菌产生的肽的质量与肠菌素A和B的质量一致。所鉴定的产细菌素肠球菌的无细胞上清液在宽范围的pH和热处理下具有相同的活性，使其成为生物保存中潜在应用的优秀候选物。测试了这些菌株产生的细菌素控制加工后污染和L.在冷藏过程中的人工污染的新鲜乳清奶酪的单核细胞增生。一种菌株被认为是杀菌的，而其他两种被归类为抑菌的。(C)2017爱思唯尔有限公司版权所有
A total of 79 bacterial strains, previously isolated from donkey milk, were screened for their antimicrobial activity against several spoilage and foodborne pathogenic bacteria. Amongst them, 3 strains belonging to Enterococcus faecium displayed antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus. Mass spectrometry analysis demonstrated that all enterococci used in this study produced peptides with masses consistent with those for enterocins A and B. The cell-free supernatants of the identified bacteriocin-producing enterococci were equally active over a wide range of pH and heat treatments making them excellent candidates for potential applications in bio-preservation. Bacteriocins produced by these strains were tested for their capability to control post-processing contamination and growth of L. monocytogenes during refrigerated storage of artificially contaminated fresh whey cheese. One strain was considered bactericidal while the other two were classified as bacteriostatic. (C) 2017 Elsevier Ltd. All rights reserved.