Synthesis and Characterization of Near-Infrared Cu-In-Se/ZnS Core/Shell Quantum Dots for In vivo Imaging
Synthesis and Characterization of Near-Infrared Cu-In-Se/ZnS Core/Shell Quantum Dots for In vivo Imaging
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DOI：
10.1021/cm101881b
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发表时间：
2010-11-23
影响因子：
8.6
通讯作者：
Dubertret, B.
中科院分区：
文献类型：
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作者：
Cassette, E.;Pons, T.;Dubertret, B.
近红外（NIR）半导体量子点（QD）代表了用于生物和生物医学成像的有前途的荧光探针。CuInSe 2是这些应用的良好候选者，这是由于其在近红外中的带隙以及与其他NIR QD材料（CdTe、CdHgTe、PbS等）相比其组分的降低的毒性。在这里，我们提出了一个简单的一锅合成路线，没有注射，以制备荧光闪锌矿Cu-In-Se核和Cu-In-Se/ZnS核/壳量子点。我们表明，所得到的核心量子点的光致发光（PL）可以调整从类似于700 nm到类似于1 μ m取决于量子点的大小（从类似于2到类似于5 nm的直径）。这些量子点的光学和结构性质是一致的电荷复合通过供体受体的水平，而不是直接激子复合。最后，我们表明，这些量子点周围的ZnS壳的生长大大增加了它们的PL量子产率（在800 nm处高达40-50%），并允许保存它们的PL特性后溶解到水中和体内，如通过检测小鼠的区域淋巴结所证明的。
Near-infrared (NIR) semiconductor quantum dots (QDs) represent promising fluorescent probes for biological and biomedical imaging. CuInSe2 is a good candidate for these applications due to its bandgap in the near-infrared and the reduced toxicity of its components compared to other NIR QD materials (CdTe, CdHgTe, PbS, etc.). Here we present a simple one-pot synthetic route without injection to make fluorescent sphalerite Cu-In-Se core and Cu-In-Se/ZnS core/shell QDs. We show that the photoluminescence (PL) of the resulting core QDs can be tuned from similar to 700 nm to similar to 1 mu m depending on the QD size (from similar to 2 to similar to 5 nm in diameter). The optical and structural properties of these QDs are consistent with charge recombination via donor acceptor levels instead of direct excitonic recombination. Finally, we show that the growth of a ZnS shell around these QDs increases their PL quantum yield substantially (up to 40-50% at 800 nm) and allows preservation of their PL properties after solubilization into water and in vivo, as demonstrated by detection of the regional lymph node in a mouse.