Three dimensional analysis of microaneurysms in the human diabetic retina
Three dimensional analysis of microaneurysms in the human diabetic retina
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DOI：
10.1046/j.1469-7580.1999.19410089.x
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发表时间：
1999-01-01
影响因子：
2.4
通讯作者：
Boulton, ME
中科院分区：
文献类型：
--
作者：
Moore, J;Bagley, S;Boulton, ME
应用免疫组织化学技术结合共聚焦激光扫描显微镜对正常人和糖尿病患者视网膜血管进行了研究。该技术，染色的血管性血友病因子，允许大面积的视网膜血管可视化和异常进行详细研究，而不干扰组织结构。在10只正常眼睛中仅观察到一个微动脉瘤，定义为任何局灶性毛细血管扩张，但在5只糖尿病视网膜中的4只中观察到许多微动脉瘤;计数在每0.41 mm（2）样本面积0到26之间变化。根据形态学将微动脉瘤分为3类：囊状、梭形和局灶性隆起。大多数是囊状的，这些没有首选的方向。大多数微动脉瘤仅与2条血管相关，表明它们不太可能在血管连接处发生。观察到大多数起源于内核层，因此位于视网膜内毛细血管丛的较深部分。微动脉瘤染色的变化可能与临床上观察到的荧光素血管造影期间染料渗漏的内皮功能障碍相关。
The retinal vasculature of postmortem normal human and diabetic eyes was studied using an immunohistochemical technique in conjunction with confocal laser scanning microscopy. The technique, which stained for von Willebrand factor, allowed both large areas of the retinal vasculature to be visualised and abnormalities to be studied in detail without disturbing the tissue architecture. Only one microaneurysm, defined as any focal capillary dilation, was observed in 10 normal eyes but numerous microaneurysms were seen in 4 out of 5 diabetic retinas; counts varied between 0 and 26 per 0.41 mm(2) sample area. Microaneurysms were classified into 3 categories according to morphology: saccular, fusiform and focal bulges. Most were saccular, these having no preferred orientation. The majority of microaneurysms were associated with just 2 vessels suggesting they were unlikely to develop at vascular junctions. The majority were observed to originate from the inner nuclear layer and were therefore in the deeper part of the inner retinal capillary plexus. Variation in the staining of microaneurysms may correlate with endothelial dysfunction seen clinically as dye leakage during fluorescein angiography.