Characterization and analysis of three newly isolated hexachlorocyclohexane (HCH)-degrading strains
Characterization and analysis of three newly isolated hexachlorocyclohexane (HCH)-degrading strains
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DOI：
10.1016/j.ibiod.2013.08.023
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发表时间：
2013-11
影响因子：
4.8
通讯作者：
Li Cao;Jianhong Xu;Mingxing Li;Guangyan Wu;Jun Wang;Yi-Ting Guan;Jian He;Shun-Peng Li;Q. Hong
中科院分区：
文献类型：
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作者：
Li Cao;Jianhong Xu;Mingxing Li;Guangyan Wu;Jun Wang;Yi-Ting Guan;Jian He;Shun-Peng Li;Q. Hong
分离并鉴定了3株降解γ-六氯环己烷（γ-HCH）的细菌（BHC-B、BHC-C和BHC-D）。经鉴定，它们均为Sphingobiumsp.根据16 S rRNA基因序列和生理生化特征进行系统发育分析。首次采用ERIC-PCR技术对鞘氨醇杆菌进行了同源性分析。菌株BHC-B、BHC-C和BHC-D还能降解其他三种HCH异构体（α-、β-和δ-异构体），其降解率顺序为γ-HCH > α-HCH > δ-HCH > β-HCH。对同一异构体，三株降解菌的降解速率顺序为BHC-C > BHC-B > BHC-D。这3个菌株具有6个酮林烯（linA、linB、linC、linD、linE、linR）。linA、linC、linD、linE和linR的序列相似性为99%~ 100%，在氨基酸水平上高度保守。而LinB基因序列差异较大，约为2%~ 3%，这可能是不同菌株对同一HCH异构体降解速率差异的原因。然而，需要进一步的研究来证明这一假设。
Three bacterial strains (designated as BHC-B, BHC-C, and BHC-D) that degrade γ-hexachlorocyclohexane (γ-HCH) were isolated and characterized. They were all identified asSphingobiumsp. according to the phylogenetic analysis of the 16S rRNA gene sequence and the physiologic and biochemical characteristics. Analysis with ERIC–PCR was first used to determine the similarities of the differentSphingobiumstrains. Strains BHC-B, BHC-C, and BHC-D could also degrade three other HCH isomers (α-, β-, and δ-isomer), and the degradation rates were in the following order: γ-HCH > α-HCH > δ-HCH > β-HCH. For the same isomer, the degradation rates of the three HCH-degrading strains were in the order: BHC-C > BHC-B > BHC-D. These three strains had six keylingenes (linA,linB,linC,linD,linE,linR). The sequence similarities oflinA,linC,linD,linE, andlinRwere 99%–100% and highly conserved at the amino acid level. By contrast, the LinB sequence diverged by 2%–3%, which might be responsible for the difference of the degradation rate in different strains against the same HCH isomer. However, further studies are needed to prove this hypothesis.