Viral kinetics are associated with changes in cytokines and chemokines in serum and target organs of SSM-CVB3-infected macaques
Viral kinetics are associated with changes in cytokines and chemokines in serum and target organs of SSM-CVB3-infected macaques
复制标题
病毒动力学与 SSM-CVB3 感染的猕猴血清和靶器官中细胞因子和趋化因子的变化有关
DOI：
10.1016/j.yexmp.2012.06.006
复制
发表时间：
2013-02-01
影响因子：
3.6
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Han, Tiesuo;Zhao, Kui;Gao, Feng
Objective: To determine the relationship between viral kinetics and the expression patterns for different cytokines and chemokines in the serum and organs of coxsackievirus B3 (SSM-CVB3)-infected macaques over the course of infection.Methods: SSM-CVB3 levels in serum and organs were measured using the Spearman-Karber 50% tissue culture infectious dose (TCID50) method. Cytokine and chemokine levels in the serum and organs were measured by indirect-ELISA.Results: Low viral titers were detected in the serum samples on the first day post-inoculation (p.i.) and peaked at 6 to 10 days p.i. in the serum samples from five macaques. Serum levels of IL-1 beta, IL-2, IL-6, IL-12p40, IL-17 gamma, TNF-alpha, MCP-1 and MIP-1 beta were detected each day and, similar to the viral titers, peaked at 6 to 10 days. IL-10 was only detected on days 10 to 14 p.i. Additionally, higher viral titers and relative viral mRNA levels were associated with higher cytokine and chemokine levels in selected tissues from infected macaques including heart, liver, spleen, lung, kidney and brain.Conclusion: The results indicate that patterns of cytokine and chemokine response are associated with viral kinetics in the serum and target organs of SSM-CVB3-infected macaques, suggesting that the changes in cytokines and chemokines could help further our understanding of the progress of CVB3 infections in clinical settings. (C) 2012 Elsevier Inc. All rights reserved.
