ISOLATION AND CHARACTERIZATION OF THE ACTIVE CDNA OF THE HUMAN CELL-CYCLE GENE (RCC1) INVOLVED IN THE REGULATION OF ONSET OF CHROMOSOME CONDENSATION
ISOLATION AND CHARACTERIZATION OF THE ACTIVE CDNA OF THE HUMAN CELL-CYCLE GENE (RCC1) INVOLVED IN THE REGULATION OF ONSET OF CHROMOSOME CONDENSATION
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DOI：
10.1101/gad.1.6.585
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发表时间：
1987-08-01
影响因子：
10.5
通讯作者：
NISHIMOTO, T
中科院分区：
文献类型：
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作者：
OHTSUBO, M;KAI, R;NISHIMOTO, T
在DNA介导的基因转移到tsBN 2细胞系中后，克隆了人RCC 1基因，tsBN 2细胞系在非允许温度（39.5-40 ℃）下显示出过早的染色体凝聚。该基因编码一个2.5 kb的poly（A）+ RNA，在仓鼠和人类中非常保守。我们从Okayama-Berg人cDNA文库中分离出15个cDNA克隆，并发现两个可以与基因组DNA克隆的效率相当地补充tsBN 2突变。这两个活性cDNA克隆的碱基序列在5'近端不同，但都具有共同的开放阅读框，编码421个氨基酸的蛋白质，计算分子量为44，847，具有7个约60个氨基酸的同源重复结构域。使用分选的染色体片段将该人RCC 1基因定位于人1号染色体。
The human RCC1 gene was cloned after DNA-mediated gene transfer into the tsBN2 cell line, which shows premature chromosome condensation at nonpermissive temperatures (39.5-40 degrees C). This gene codes for a 2.5-kb poly(A)+ RNA that is well conserved in hamsters and humans. We isolated 15 cDNA clones from the Okayama-Berg human cDNA library, and found two that can complement the tsBN2 mutation with an efficiency comparable to that of the genomic DNA clone. The base sequences of these two active cDNA clones differ at the 5' proximal end, yet both have a common open reading frame, encoding a protein of 421 amino acids with a calculated molecular weight of 44,847 and with seven homologous repeated domains of about 60 amino acids. This human RCC1 gene was located to human chromosome 1 using sorted chromosomal fractions.