Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction
Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction
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DOI：
10.1016/j.neuron.2004.08.019
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发表时间：
2004-09-02
期刊：
影响因子：
16.2
通讯作者：
Vosshall, LB
中科院分区：
文献类型：
--
作者：
Larsson, MC;Domingos, AI;Vosshall, LB
果蝇被各种各样的气味所吸引，这些气味表明食物、潜在配偶或诱人的产卵地点的存在。大多数果蝇嗅觉神经元表达两种气味受体基因：Or83b，一种功能未知的广泛表达的受体，以及61个选择性表达受体家族中的一个或多个成员。虽然传统的气味受体高度分化，但Or83b在昆虫物种之间非常保守。有两个模型可以解释Or83b的功能：它可以独立于传统的气味受体与特定的气味刺激相互作用，或者它可以与这些受体协同作用，介导对所有气味的反应。我们的结果支持第二个模型。在Or83b突变体中，传统气味受体的树突定位被取消。与这种细胞缺陷相一致的是，Or83b突变扰乱了对许多气味的行为和电生理反应。因此，Or83b编码了一种非典型的气味受体，它在嗅觉中起着重要的普遍作用。
Fruit flies are attracted by a diversity of odors that signal the presence of food, potential mates, or attractive egg-laying sites. Most Drosophila olfactory neurons express two types of odorant receptor genes: Or83b, a broadly expressed receptor of unknown function, and one or more members of a family of 61 selectively expressed receptors. While the conventional odorant receptors are highly divergent, Or83b is remarkably conserved between insect species. Two models could account for Or83b function: it could interact with specific odor stimuli independent of conventional odorant receptors, or it could act in concert with these receptors to mediate responses to all odors. Our results support the second model. Dendritic localization of conventional odorant receptors is abolished in Or83b mutants. Consistent with this cellular defect, the Or83b mutation disrupts behavioral and electrophysiological responses to many odorants. Or83b therefore encodes an atypical odorant receptor that plays an essential general role in olfaction.