Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes.
Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes.
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人类免疫缺陷病毒 1 型 Nef 与 T 淋巴细胞中的细胞丝氨酸激酶相关。
DOI：
10.1073/pnas.91.4.1539
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发表时间：
1994
影响因子：
11.1
通讯作者：
Cheng-Mayer,C
中科院分区：
文献类型：
--
作者：
Sawai,ET;Baur,A;Struble,H;Peterlin,BM;Levy,JA;Cheng-Mayer,C
通过以混合 CD8-Nef 融合蛋白的形式组成型表达来自人类免疫缺陷病毒 1 型 (HIV-1SF2) SF2 株的 Nef 的 T 细胞系或长期感染 HIV-1SF2 的 T 细胞系，发现了一种与 Nef 特异性相关的细胞丝氨酸激酶。与 Nef 免疫共沉淀的 62 kDa 和 72 kDa 蛋白质在体外激酶测定中被磷酸化。这种与 Nef 相关的丝氨酸激酶活性不会被蛋白激酶 C 或蛋白激酶 A 的抑制剂阻断，并且当 Nef 在氨基酸 94 或 99 处被截短时会丢失。这些发现证明丝氨酸激酶活性与人 T 淋巴细胞中表达的 Nef 相关。
With T-cell lines constitutively expressing Nef from the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in the form of a hybrid CD8-Nef fusion protein or T-cell lines chronically infected with HIV-1SF2, a cellular serine kinase was found that specifically associates with Nef. Proteins of 62 kDa and 72 kDa, which coimmunoprecipitated with Nef, were phosphorylated in in vitro kinase assays. This Nef-associated serine kinase activity was not blocked by inhibitors of protein kinase C or protein kinase A and was lost when Nef was truncated at amino acid 94 or 99. These findings present evidence that a serine kinase activity is associated with Nef expressed in human T lymphocytes.