Using cloud computing infrastructure with CloudBioLinux, CloudMan, and Galaxy.
Using cloud computing infrastructure with CloudBioLinux, CloudMan, and Galaxy.
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DOI：
10.1002/0471250953.bi1109s38
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发表时间：
2012-06
影响因子：
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通讯作者：
Taylor, James
中科院分区：
文献类型：
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作者：
Afgan, Enis;Chapman, Brad;Jadan, Margita;Franke, Vedran;Taylor, James
云计算彻底改变了可用性，并访问了计算和存储资源。可以在Web浏览器中只需单击几下即可为大型计算基础架构提供大型计算基础架构。但是，这些资源通常以低级基础架构组件的形式提供，在使用前需要采购和配置。在此协议中，我们演示了如何利用云计算资源来执行开放式生物信息学分析，并通过对基础云基础架构进行完全自动化的管理。通过将三个项目结合在一起，即CloudBiolinux，Cloudman和Galaxy与一个有凝聚力的单元相结合，我们使研究人员能够访问在灵活的云计算基础结构之上的100多个参考基因组的预配置的生物信息学工具和千兆字节。该协议演示了如何通过图形桌面接口，并行命令行接口和基于Web的Galaxy接口来设置可用的基础结构以及如何通过图形桌面接口使用工具。
Cloud computing has revolutionized availability and access to computing and storage resources; making it possible to provision a large computational infrastructure with only a few clicks in a web browser. However, those resources are typically provided in the form of low-level infrastructure components that need to be procured and configured before use. In this protocol, we demonstrate how to utilize cloud computing resources to perform open-ended bioinformatics analyses, with fully automated management of the underlying cloud infrastructure. By combining three projects, CloudBioLinux, CloudMan, and Galaxy into a cohesive unit, we have enabled researchers to gain access to more than 100 preconfigured bioinformatics tools and gigabytes of reference genomes on top of the flexible cloud computing infrastructure. The protocol demonstrates how to setup the available infrastructure and how to use the tools via a graphical desktop interface, a parallel command line interface, and the web-based Galaxy interface.