Exploiting the ubiquitin and phosphoinositide pathways by the Legionella pneumophila effector, SidC.
Exploiting the ubiquitin and phosphoinositide pathways by the Legionella pneumophila effector, SidC.
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DOI：
10.1007/s00294-015-0521-y
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发表时间：
2016-02
期刊：
影响因子：
2.5
通讯作者：
Mao Y
中科院分区：
文献类型：
--
作者：
Wasilko DJ;Mao Y
细胞内细菌病原体使用分泌的效应蛋白来改变宿主细胞过程，目的是破坏宿主防御并允许感染进展。一种这样的病原体，嗜肺军团菌，分泌约300种这样的蛋白质进入其宿主，以改变许多途径，包括细胞内运输，磷酸肌醇代谢和细胞信号传导。以前发现军团菌效应子SidC与PI（4）P结合，并负责ER蛋白以及泛素化物种富集到含军团菌的空泡中。通过我们最近的工作，我们发现SidC含有一个独特的N端E3泛素连接酶结构域和一个C端新的PI（4）P结合结构域。我们的研究结果表明，SidC连接两个不同的细胞途径，泛素和磷酸肌醇。然而，泛素连接酶活性如何调节宿主膜运输事件仍有待研究。
Intracellular bacterial pathogens use secreted effector proteins to alter host cellular processes, with the goal of subverting host defenses and allowing the infection to progress. One such pathogen, Legionella pneumophia, secretes ~300 such proteins into its host to alter a number of pathways including intracellular trafficking, phosphoinositide metabolism, and cell signaling. The Legionella effector SidC was previously found to bind to PI(4)P and was responsible for the enrichment of ER proteins as well as ubiquitinated species to the Legionella-containing vacuoles. Through our recent work, we have discovered that SidC contains a unique N-terminal E3 ubiquitin ligase domain and a C-terminal novel PI(4)P-binding domain. Our results demonstrate that SidC serves to link two distinct cellular pathways, ubiquitin and phosphoinositide. However, how the ubiquitin ligase activity regulates host membrane trafficking events remain to be investigated.