Cocaine and methamphetamine induce opposing changes in BOLD signal response in rats.
Cocaine and methamphetamine induce opposing changes in BOLD signal response in rats.
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DOI：
10.1016/j.brainres.2016.04.040
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发表时间：
2016-07-01
期刊：
影响因子：
2.9
通讯作者：
Reichel CM
中科院分区：
文献类型：
--
作者：
Taheri S;Xun Z;See RE;Joseph JE;Reichel CM
对精神兴奋剂成瘾者的神经影像学研究已经报道了复吸所涉及的脑区的功能性神经活动变化。然而，精神兴奋剂甲基苯丙胺和可卡因在类似环境中对神经元活动的影响之间的差异尚未澄清。由于人类研究受到无法研究精神兴奋剂药物的初始影响的限制，我们在大鼠模型中解决了这个问题。在这里，我们报告甲基苯丙胺和可卡因诱导的血氧水平依赖（BOLD）的信号变化，使用功能磁共振成像（fMRI）在大鼠接受药物的第一次在成像会议。23只雄性Long Evans大鼠接受了fMRI成像，并接受了甲基苯丙胺、可卡因或生理盐水的静脉输注。在7 T BioSpec专用研究MR扫描仪上在异氟烷气体（1.5-2%）下进行解剖和药理学fMRI（pfMRI）。在收集基线数据10分钟后，大鼠在接下来的10分钟内接受药物，总共扫描40分钟。然后对数据进行预处理，并在解剖学定义的感兴趣区域（ROI）中进行统计分析，这些区域与持续的药物寻求和复发有关。甲基苯丙胺在成像过程中导致持续的负BOLD信号变化的关键区域的复发电路，除了前额叶皮层。相比之下，可卡因在这些相同的区域诱发了阳性或不变的BOLD信号。在所有研究的ROI中，使用生理盐水后BOLD信号没有变化。急性甲基苯丙胺和可卡因有不同的模式的功能活动所测量的pfMRI。
Neuroimaging studies in psychostimulant addicts have reported functional neural activity changes in brain regions involved in relapse. However, the difference between the effects of the psychostimulants methamphetamine and cocaine on neuronal activity in a similar setting not been clarified. Since studies in humans are limited by the inability to study the initial impact of psychostimulant drugs, we addressed this issue in a rat model. Here, we report methamphetamine and cocaine-induced blood-oxygen-level dependent (BOLD) signal change using functional magnetic resonance imaging (fMRI) in rats receiving drug for the first time during the imaging session. Twenty-three male Long Evans rats underwent fMRI imaging and received an intravenous infusion of methamphetamine, cocaine, or saline. Anatomical and pharmacological fMRI (pfMRI) were performed on a 7T BioSpec dedicated research MR scanner under isoflurane gas (1.5-2%). After collecting baseline data for 10 min, rats received drug over the next 10 min for a total 40 min scan time. Data were then preprocessed and statistically analyzed in anatomically defined regions of interest (ROIs) that have been implicated in persistent drug seeking and relapse. Methamphetamine during the imaging session resulted in a sustained negative BOLD signal change in key regions of the relapse circuit, except for the prefrontal cortex. In contrast, cocaine evoked a positive or unchanged BOLD signal in these same regions. In all of the investigated ROIs, there were no changes in BOLD signal following saline. Acute methamphetamine and cocaine have distinct patterns of functional activity as measured by pfMRI.