Photodissociation Spectroscopy of Hydrated Myoglobin Ions Isolated by IR-laser Ablation of a Droplet Beam: Recovery from pH-denatured Structure by Gas-phase Isolation
Photodissociation Spectroscopy of Hydrated Myoglobin Ions Isolated by IR-laser Ablation of a Droplet Beam: Recovery from pH-denatured Structure by Gas-phase Isolation
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DOI：
10.1246/cl.180884
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发表时间：
2019-02-01
影响因子：
1.6
通讯作者：
Kohno, Jun-ya
中科院分区：
文献类型：
--
作者：
Asami, Hiroya;Kawabata, Rina;Kohno, Jun-ya
在Soret区附近获得了气相分离的水合肌红蛋白（Mb）离子的光解光谱。相对于酸性水溶液的光谱有较大的红移，表明从ph变性状态到孤立的天然结构发生了显著的结构变化。因此，Mb的pH变性受到周围水分子的强烈影响。
Photodissociation spectra near the Soret region were acquired for hydrated myoglobin (Mb) ions isolated in the gas phase. The spectra had a large red shift relative to that from an acidic aqueous solution, which indicated a significant structural change from a pH-denatured state to the isolated native structure. The pH denaturation of Mb was therefore strongly affected by surrounding water molecules.