Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae
Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae
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DOI：
10.1007/s00705-008-0288-2
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Pankovics, Peter
中科院分区：
文献类型：
--
作者：
Reuter, Gabor;Boldizsar, Akos;Pankovics, Peter
柯布病毒是小核糖核酸病毒科的一个新属。目前已知的有两种：爱知病毒（人科布病毒）和牛科布病毒（U-1）。本研究测定了猪科布病毒（kobuvirus /swine/S-1-HUN/2007/Hungary, EU787450）的全核苷酸、氨基酸序列和遗传组织。S-1-HUN基因组的vpg -5' utr先导蛋白-结构蛋白（VP0、VP3、VP1）-非结构蛋白（2A-2C、3A-3D）-3' utr聚(A)尾结构是小核糖核酸病毒的典型结构。8210核苷酸（nt）长的RNA基因组包含一个大的开放阅读框（7467 nt），编码2488个氨基酸（aa）的潜在多蛋白前体，分别与爱知病毒和U-1具有57/56%和63/64%的nt/aa同源性。5'UTR包含一个肝病毒/鼠疫病毒样内部核糖体进入位点（IRES IV型b类），具有保守的假结、II和IIIa-f结构域。在2B中检测到一个串联重复序列（一个30个氨基酸长的基序）。在测试猪场60个6月龄以下猪的粪便样本中，39个（65%）呈阳性（3周龄以下的发病率为90%）。猪科布病毒属于科布病毒属的第三个潜在新种。
Kobuvirus is a new genus in the family Picornaviridae. Two species are currently known: Aichi virus (human kobuvirus) and Bovine kobuvirus (U-1). In this study, the complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus (Kobuvirus/swine/S-1-HUN/2007/Hungary, EU787450) were determined. The structure of the S-1-HUN genome, VPg-5'UTR-leader protein-structural proteins (VP0, VP3, VP1)-non-structural proteins (2A-2C, 3A-3D)-3'UTR-poly(A) tail, was found to be typical of picornavirus. The 8210-nucleotide (nt)-long RNA genome contains a large open reading frame (7467 nt) encoding a potential polyprotein precursor of 2488 amino acids (aa) that has 57/56% and 63/64% nt/aa identity with Aichi virus and U-1, respectively. The 5'UTR contains a hepacivirus/pestivirus-like internal ribosomal entry site (IRES type IV group-B-like) with conserved pseudoknot, II and IIIa-f domains. A tandem repeat (a 30-amino-acid-long motif) was detected in 2B. Thirty-nine (65%) of the 60 fecal samples from pigs under the age of 6 months at the tested farm were positive (the incidence was 90% under the age of 3 weeks). Porcine kobuvirus belongs to a potential new species-the third-in the genus Kobuvirus.