Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).
Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).
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DOI：
10.1016/j.xpro.2021.100686
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Gonen T
中科院分区：
文献类型：
--
作者：
Martynowycz MW;Gonen T
We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to. Protocol for focused ion-beam milling of protein crystals for MicroED experiments Detailed steps to generate crystalline lamellae Procedures for MicroED data collection Optimization and troubleshooting for highest resolution possible We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED.
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DOI：
10.1107/s0907444910045749
发表时间：
2011-04
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Winn MD;Ballard CC;Cowtan KD;Dodson EJ;Emsley P;Evans PR;Keegan RM;Krissinel EB;Leslie AG;McCoy A;McNicholas SJ;Murshudov GN;Pannu NS;Potterton EA;Powell HR;Read RJ;Vagin A;Wilson KS
通讯作者：
Wilson KS
影响因子：
5.7
作者：
Martynowycz, Michael W.;Zhao, Wei;Gonen, Tamir
通讯作者：
Gonen, Tamir
影响因子：
5
作者：
Beale, Emma, V;Waterman, David G.;Zhang, Peijun
通讯作者：
Zhang, Peijun
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.8
作者：
Shi, Dan;Nannenga, Brent L.;Gonen, Tamir
通讯作者：
Gonen, Tamir