The Impact of Multiparity on Uterine Gene Expression and Decidualization in Mice
The Impact of Multiparity on Uterine Gene Expression and Decidualization in Mice
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多胎对小鼠子宫基因表达和蜕膜化的影响
DOI：
10.1177/1933719115612131
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发表时间：
2016-05-01
影响因子：
2.9
通讯作者：
Fu，Yong-Sheng
中科院分区：
文献类型：
--
作者：
Liu，Ji-Long;Zuo，Ru-Juan;Fu，Yong-Sheng
已经确定的是，以前的怀孕对以后的怀孕有好处。然而，根本的机制尚未确定。我们推测，多产可能会影响蜕膜化过程中早期妊娠。为了验证这一假设，我们使用RNA测序（RNA-seq）分析了小鼠子宫中与多产相关的全局基因变化。我们共鉴定了131个差异表达基因（倍数变化> 2，错误发现率< 0.05），其中58个基因在第二次妊娠（SP）中与第一次妊娠相比下调，73个基因上调。功能聚类分析显示，参与应激反应的基因显著富集。最重要的是，差异表达基因的显着部分，14个基因或10.7%，与蜕膜化相关的基因列表重叠。定量逆转录（RT）聚合酶链反应（qRT-PCR）分析证实了4个基因的表达降低（Klk 1，激肽释放酶1; H2-Eb 1，组织相容性2 II类抗原E β; Mmp 7，基质金属肽酶7; Pdpn，podoplanin）和2种基因表达增加（Thy 1，胸腺细胞抗原1; Ptgs 2，胰高血糖素内过氧化物合酶2）在SP。除了蛋白质编码基因，我们还确定了差异表达的长非编码RNA AI 506816。我们的数据提供了新的见解的分子机制的有利影响多胎。
It has been well established that a previous pregnancy exhibits a beneficial effect on the subsequent pregnancy. However, the underlying mechanisms have not been defined. We hypothesized that multiparity may affect decidualization process during early pregnancy. To test this hypothesis, we analyzed global gene changes associated with multiparity in the mouse uterus using RNA-sequencing (RNA-seq). We identified a total of 131 differentially expressed genes (fold change > 2 and false discovery rate < 0.05), of which 58 were downregulated and 73 genes were upregulated in the second pregnancy (SP) compared to the first pregnancy. Functional clustering analysis showed that genes involved in stress response were significantly enriched. Most importantly, a significant portion of differentially expressed genes, 14 genes or 10.7%, overlapped with the gene list associated with decidualization. Quantitative reverse transcription (RT) polymerase chain reaction (qRT-PCR) analysis confirmed a decreased expression of 4 genes (Klk1, kallikrein 1; H2-Eb1, histocompatibility 2 class II antigen E beta; Mmp7, matrix metallopeptidase 7; Pdpn, podoplanin) and an increase in expression of 2 genes (Thy1, thymus cell antigen 1; Ptgs2, prostaglandin-endoperoxide synthase 2) in SP. Beyond protein-coding genes, we also identified a differentially expressed long noncoding RNA AI506816. Our data provide new insights into the molecular mechanisms underlying the beneficial effect of multiparity.