Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates
Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates
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DOI：
10.1016/j.peptides.2012.09.017
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发表时间：
2012-12-01
期刊：
影响因子：
3
通讯作者：
Liu, Shuying
中科院分区：
文献类型：
--
作者：
Chen, Ning;Yang, Hongmei;Liu, Shuying
采用3种不同的蛋白酶分别水解核桃蛋白，制备抗氧化肽。采用1，1-二苯基-2-苦基肼（DPPH）法测定酶解产物的抗氧化活性。在酶解产物中，3 h酶解产物的抗氧化活性最高，同时具有淬灭羟自由基、螯合亚铁离子、还原力和抑制脂质过氧化的作用。然后，3-h的胃蛋白酶水解产物依次通过超滤，凝胶过滤和RP-HPLC纯化。经RP-HPLC-ESI-MS分析，抗氧化活性最高的肽段序列为Ala-Asp-Ala-Phe（42323 Da），这是首次从核桃蛋白酶解物中鉴定出。最后，该肽对脂质过氧化的抑制作用与还原型谷胱甘肽（GSH）相似。这些结果表明，蛋白质水解物和/或其分离肽可以有效地用作食品添加剂。(C)2012 Elsevier Inc. All rights reserved.
Walnut proteins were hydrolyzed separately using three different proteases to obtain antioxidant peptides. The antioxidant activities of the hydrolysates were measured using 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. Among hydrolysates, pepsin hydrolysate obtained by 3h exhibited the highest antioxidant activities, which could also quench the hydroxyl radical, chelate ferrous ion, exhibit reducing power and inhibit the lipid peroxidation. Then, 3-h pepsin hydrolysates were purified sequentially by ultrafiltration, gel filtration and RP-HPLC. The sequence of the peptide with the highest antioxidative activity was identified to be Ala-Asp-Ala-Phe (42323 Da) using RP-HPLC-ESI-MS, which was identified for the first time from walnut protein hydrolysates. Last, the inhibition of the peptide on lipid peroxidation was similar with that of reduced glutathione (GSH). These results indicate that the protein hydrolysates and/or its isolated peptides may be effectively used as food additives. (C) 2012 Elsevier Inc. All rights reserved.