Identification of genes differentially expressed in osteoclast-like cells
Identification of genes differentially expressed in osteoclast-like cells
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DOI：
10.1089/jir.2005.25.227
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发表时间：
2005-04-01
影响因子：
2.3
通讯作者：
Kukita, T
中科院分区：
文献类型：
--
作者：
Nomiyama, H;Egami, K;Kukita, T
骨骼系统的稳态是通过骨形成和吸收之间的平衡来维持的。 NF-κ B 配体受体激活剂 (RANKL) 诱导骨吸收细胞、破骨细胞的分化。为了鉴定破骨细胞分化过程中调控的基因，我们使用小鼠 RAW264 巨噬细胞系构建了消减 cDNA 文库，该细胞系在 RANKL 处理后分化为破骨细胞样多核细胞。 Northern 印迹分析显示 RANKL 处理上调了 17 个基因的表达。其中包括五个H+-ATP酶亚基、两个趋化因子和破骨细胞标记组织蛋白酶K的基因。此外，诱导基因中还包括最近揭示的人树突状细胞(DC)特异性跨膜蛋白(DC-STAMP)的小鼠同源物，其在破骨细胞生成中的功能。这些诱导基因的表征将有助于深入了解破骨细胞的生物学和骨相关疾病的机制。
Homeostasis of the skeletal system is maintained by a balance between bone formation and resorption. The receptor activator of NF-kappa B ligand ( RANKL) induces the differentiation of bone-resorbing cells, osteoclasts. To identify genes regulated during osteoclast differentiation, we constructed a subtraction cDNA library using a mouse RAW264 macrophage cell line that differentiates into osteoclast-like multinucleated cells after treatment with RANKL. Northern blot analysis showed that RANKL treatment upregulated expression of 17 genes. Among these were the genes for five H+-ATPase subunits, two chemokines, and the osteoclast marker cathepsin K. In addition, a mouse homolog of human dendritic cell (DC)-specific transmembrane protein (DC-STAMP), whose function in osteoclastogenesis was recently revealed, was also included in the induced genes. Characterization of these inducible genes will provide an insight into the biology of osteoclasts and the mechanism of bone-related diseases.