TRIM5 alpha cytoplasmic bodies are highly dynamic structures.
TRIM5 alpha cytoplasmic bodies are highly dynamic structures.
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DOI：
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发表时间：
2007
影响因子：
3.3
通讯作者：
E. Campbell;M. P. Dodding;M. Yap;Xiaolu Wu;S. Gallois-Montbrun;M. Malim;J. Stoye;T. Hope
中科院分区：
文献类型：
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作者：
E. Campbell;M. P. Dodding;M. Yap;Xiaolu Wu;S. Gallois-Montbrun;M. Malim;J. Stoye;T. Hope
三分基序（TRIM）5 α最近被鉴定为宿主限制因子，其具有以物种依赖性方式阻断某些逆转录病毒感染的能力。这种蛋白质的一个有趣的特征是，它定位于被称为胞质体的不同的胞质簇中。这些胞质体在TRIM5 α功能中的潜在作用仍有待确定。通过使用荧光融合蛋白和活细胞显微镜，我们研究了TRIM5 α胞质体的定位和动力学。这种分析表明，细胞质体是高度移动的，表现出短跳跃运动和单向长距离运动沿着微管网络。细胞质体的形态也是动态的。最后，光漂白和光活化分析表明，存在于细胞质体中的TRIM5 α蛋白是非常动态的，在细胞质体和更扩散的细胞质群体之间快速交换。因此，TRIM5 α胞质体是与功能或调节作用更一致的动态结构，而不是代表死端静态结构的蛋白质聚集体或包涵体。
Tripartite motif (TRIM)5 alpha has recently been identified as a host restriction factor that has the ability to block infection by certain retroviruses in a species-dependent manner. One interesting feature of this protein is that it is localized in distinct cytoplasmic clusters designated as cytoplasmic bodies. The potential role of these cytoplasmic bodies in TRIM5 alpha function remains to be defined. By using fluorescent fusion proteins and live cell microscopy, we studied the localization and dynamics of TRIM5 alpha cytoplasmic bodies. This analysis reveals that cytoplasmic bodies are highly mobile, exhibiting both short saltatory movements and unidirectional long-distance movements along the microtubule network. The morphology of the cytoplasmic bodies is also dynamic. Finally, photobleaching and photoactivation analysis reveals that the TRIM5 alpha protein present in the cytoplasmic bodies is very dynamic, rapidly exchanging between cytoplasmic bodies and a more diffuse cytoplasmic population. Therefore, TRIM5 alpha cytoplasmic bodies are dynamic structures more consistent with a role in function or regulation rather than protein aggregates or inclusion bodies that represent dead-end static structures.