ACUTE EFFECTS OF 3,4-METHYLENEDIOXYMETHAMPHETAMINE (MDMA) ON MONOAMINES IN RAT CAUDATE
ACUTE EFFECTS OF 3,4-METHYLENEDIOXYMETHAMPHETAMINE (MDMA) ON MONOAMINES IN RAT CAUDATE
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DOI：
10.1016/0091-3057(91)90137-q
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发表时间：
1991-07-01
影响因子：
3.6
通讯作者：
HOLSON, RR
中科院分区：
文献类型：
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作者：
GOUGH, B;ALI, SF;HOLSON, RR
采用微透析和高效液相色谱-电化学检测法（HPLC-EC）测定自由活动大鼠尾状核细胞外多巴胺（DA）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、5-羟吲哚乙酸（5-HIAA）和5-羟色胺（5-HT）的含量变化。 腹膜内（IP）注射MDMA（10 mg/kg）后，以20分钟间隔测定透析液，持续4小时。 在另一项确定MDMA对上述神经化学物质总尾状核水平影响的研究中，对动物IP注射MDMA（10 mg/kg），然后在给药后20、60、120和180分钟处死。 迅速取出大脑，并解剖尾状核，使用HPLC-EC进行神经化学分析。 MDMA引起的安非他明样增加DA释放，其次是增加DA含量。 匀浆中DOPAC和HVA均减少。 5-HT释放也增加，随后在尾状核匀浆水平下降了三个小时。 在80分钟时，DA细胞外含量为对照的686%;在120分钟时，尾状核匀浆水平为122%。 5-HT细胞外释放在20分钟时为123%，然后下降。 可以得出结论，MDMA对尾状核的急性效应至少与DA对5-HT系统的急性效应一样大。
Extracellular levels of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) were assayed in the caudate of freely moving rats using microdialysis and high performance liquid chromatography with electrochemical detection (HPLC-EC) to detect changes in their release. Dialysates were assayed at 20-minute intervals for four hours after an intraperitoneal (IP) injection of MDMA (10 mg/kg). In a separate study to determine MDMA effects on total caudate levels of the above neurochemicals, animals were injected IP with MDMA (10 mg/kg) and then sacrificed at 20, 60, 120 and 180 minutes after treatment. Brains were quickly removed, and caudate nuclei were dissected for neurochemical analysis using HPLC-EC. MDMA elicited an amphetamine-like increase in DA release, followed by an increase in DA content. DOPAC and HVA were both reduced in homogenate. 5-HT release was also increased, followed by a drop in caudate homogenate levels by three hours. DA extracellular content was 686% of control at 80 minutes; caudate homogenate levels were 122% at 120 minutes. 5-HT extracellular release was 123% at 20 minutes, then decreased thereafter. It is concluded that the acute effect of MDMA on caudate is at least as great on the DA as it is on the 5-HT system.